دورية أكاديمية
MicroRNA-191 triggers keratinocytes senescence by SATB1 and CDK6 downregulation
| العنوان: | MicroRNA-191 triggers keratinocytes senescence by SATB1 and CDK6 downregulation |
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| المؤلفون: | LENA, ANNA MARIA, MELINO, GENNARO, CANDI, ELEONORA, Mancini, M, Rivetti di Val Cervo, P, Saintigny, G, Mahé, C |
| المساهمون: | Lena, Am, Mancini, M, Rivetti di Val Cervo, P, Saintigny, G, Mahé, C, Melino, G, Candi, E |
| بيانات النشر: | Elsevier Science Limited |
| سنة النشر: | 2012 |
| المجموعة: | Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
| مصطلحات موضوعية: | Matrix Attachment Region Binding Protein, MicroRNA, Gene Silencing, Human, Cyclin-Dependent Kinase 6, Base Sequence, Down-Regulation, Cell Aging, Molecular Sequence Data, 3' Untranslated Region, Keratinocyte, Cell Cycle, Cell Line, Settore BIO/11 - BIOLOGIA MOLECOLARE |
| الوصف: | Keratinocyte replicative senescence has an important role in time-dependent changes of the epidermis, a tissue with high turnover. Senescence encompasses growth arrest during which cells remain metabolically active but acquire a typical enlarged, vacuolar and flattened morphology. It is also accompanied by the expression of endogenous senescence-associated-β-galactosidase and specific gene expression profiles. MicroRNAs levels have been shown to be modulated during keratinocytes senescence, playing key roles in inhibiting proliferation and in the acquisition of senescent markers. Here, we identify miR-191 as an anti-proliferative and replicative senescence-associated miRNA in primary human keratinocytes. Its overexpression is sufficient per se to induce senescence, as evaluated by induction of several senescence-associated markers. We show that SATB1 and CDK6 3'UTRs are two miR-191 direct targets involved in this pathway. Cdk6 and Satb1 protein levels decrease during keratinocytes replicative senescence and their silencing by siRNA is able to induce a G1 block in cell cycle, accompanied by an increase in senescence-associated markers. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000306624900012; volume:423; issue:3; firstpage:509; lastpage:514; journal:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS; http://hdl.handle.net/2108/78439Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84863332537 |
| DOI: | 10.1016/j.bbrc.2012.05.153 |
| الإتاحة: | https://doi.org/10.1016/j.bbrc.2012.05.153Test http://hdl.handle.net/2108/78439Test |
| رقم الانضمام: | edsbas.AB981CCE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.bbrc.2012.05.153 |
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