دورية أكاديمية
A film-based integrated chip for gene amplification and electrochemical detection of pathogens causing foodborne illnesses
| العنوان: | A film-based integrated chip for gene amplification and electrochemical detection of pathogens causing foodborne illnesses |
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| المؤلفون: | Park, Yoo Min, Lim, Sun Young, Shin, Su Jeong, Kim, Chi Hyun, Jeong, Soon Woo, Shin, Seol Yi, Bae, Nam Ho, Lee, Seok Jae, Na, Jeongkyeong, Jung, Gyoo Yeol, Lee, Tae Jae |
| المساهمون: | Jung, Gyoo Yeol |
| بيانات النشر: | ELSEVIER SCIENCE BV |
| سنة النشر: | 2018 |
| المجموعة: | Pohang University of Science and Technology (POSTECH): Open Access System for Information Sharing (OASIS) |
| مصطلحات موضوعية: | Chains, Chemical detection, Electrophoresis, Escherichia coli, Food safety, Gene expression, Microfluidics, Pathogens, Polymerase chain reaction, Voltammetry, DNA analysis, ELectrochemical detection, Electrochemical signals, High reproducibility, Microfluidic chambers, Point-of-care testing, Square wave voltammetry, Staphylococcus aureus, Food microbiology, 4 [5 (4 methyl 1 piperazinyl)[2,5&apos, bi 1h benzimidazol] 2&apos, yl]phenol, double stranded DNA, polycarbonate, polyester, polyimide, DNA, pibenzimol, Article, colony forming unit |
| الوصف: | Given the increased interest in public hygiene due to outbreaks of food poisoning, increased emphasis has been placed on developing novel monitoring systems for point-of-care testing (POCT) to evaluate pathogens causing foodborne illnesses. Here, we demonstrate a pathogen evaluation system utilizing simple film-based microfluidics, featuring simultaneous gene amplification, solution mixing, and electrochemical detection. To minimize and integrate the various functionalities into a single chip, patterned polyimide and polyester films were mainly used on a polycarbonate housing chip, allowing simple fabrication and alignment, in contrast to conventional polymerase chain reaction, which requires a complex biosensing system at a bench-top scale. The individual integrated sensing chip could be manually fabricated in 10 min. Using the developed film-based integrated biosensing chip, the genes from the pathogens causing foodborne illnesses were simultaneously amplified based on multiple designed microfluidic chambers and Hoechst 33258, which intercalates into double-stranded DNA, to generate the electrochemical signal. The target pathogen gene was accurately analyzed by square wave voltammetry (SWV) within the 25 s, while the gel electrophoresis required about 30 min. Based on the developed integrated biosensing chip, the 1.0 x 10(1) and 1.0 x 10(2) colony-forming unit (CFU) of Staphylococcus aureus and Escherichia coli were sensitively detected with high reproducibility in the 25 s. On the basis of the significant features of the film-based molecular analysis platform, we expect that the developed sensor could be applied to the screening of various pathogens as a POCT device. (C) 2018 Elsevier B.V. All rights reserved. ; 1 ; 1 ; 0 ; scie ; scopus |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0003-2670 |
| العلاقة: | ANALYTICA CHIMICA ACTA; Chemistry, Analytical; Chemistry; https://oasis.postech.ac.kr/handle/2014.oak/94495Test; 31848; ANALYTICA CHIMICA ACTA, v.1027, pp.57 - 66; 000434012800007; 2-s2.0-85046169832 |
| DOI: | 10.1016/j.aca.2018.03.061 |
| الإتاحة: | https://doi.org/10.1016/j.aca.2018.03.061Test https://oasis.postech.ac.kr/handle/2014.oak/94495Test |
| رقم الانضمام: | edsbas.2DADD220 |
| قاعدة البيانات: | BASE |
| تدمد: | 00032670 |
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| DOI: | 10.1016/j.aca.2018.03.061 |