دورية أكاديمية
Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection
| العنوان: | Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection |
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| المؤلفون: | Valerio Del Bono, Roberto Biselli, Roberto Nisini, Lawrence D. Loomis Price, Anna Loy, Carolina Lorusso, Dante Bassetti, D'AMELIO, Raffaele |
| المساهمون: | Valerio Del, Bono, Roberto, Biselli, Roberto, Nisini, Lawrence D., Loomis Price, Anna, Loy, Carolina, Lorusso, Dante, Bassetti, D'Amelio, Raffaele |
| بيانات النشر: | ELSEVIER SCIENCE BV |
| سنة النشر: | 1998 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | hiv-antibodie, hiv-serodiagnosi, isoelectricfocusing, perinatal infection |
| الوصف: | Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection-by means of viral culture and/or PCR-or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs tall mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V-3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response. (C) 1998 Elsevier Science B.V. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/9949956; info:eu-repo/semantics/altIdentifier/wos/WOS:000078247100006; volume:11; issue:3; firstpage:203; lastpage:210; numberofpages:8; journal:JOURNAL OF CLINICAL VIROLOGY; http://hdl.handle.net/11573/14989Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0032433798; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000078247100006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest; http://www.scopus.com/inward/record.url?eid=2-s2.0-0032433798&partnerID=65&md5=fee9f2a64579f3a3917562fa2233d1b7Test |
| DOI: | 10.1016/s0928-0197(98)00050-6 |
| الإتاحة: | https://doi.org/10.1016/s0928-0197Test(98)00050-6 http://hdl.handle.net/11573/14989Test http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000078247100006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest http://www.scopus.com/inward/record.url?eid=2-s2.0-0032433798&partnerID=65&md5=fee9f2a64579f3a3917562fa2233d1b7Test |
| رقم الانضمام: | edsbas.B67C6D94 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/s0928-0197(98)00050-6 |
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