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1دورية أكاديمية
المؤلفون: Villafañe, Luciana, Rocha, Rosana Valeria, Bigi, María Mercedes, Klepp, Laura Inés, Taboga, Oscar Alberto, Forrellad, Marina Andrea, López, María Gabriela, Bigi, Fabiana
المصدر: Vet Immunol Immunopathol ; ISSN:1873-2534 ; Volume:273
مصطلحات موضوعية: Antigens, EsxG, IFN-γ assay, Mb0309, Mycobacterium bovis
الوصف: Bovine tuberculosis (bTB) represents a threat to livestock production. Mycobacterium bovis is the main causative agent of bTB and a pathogen capable of infecting wildlife and humans. Eradication programs based on surveillance in slaughterhouses with mandatory testing and culling of reactive cattle have failed to eradicate bTB in many regions worldwide. Therefore, developing effective tools to control this disease is crucial. Using a computational tool, we identified proteins in the M. bovis proteome that carry predictive binding peptides to BoLADRB3.2 and selected Mb0309, Mb1090, Mb1810 and Mb3810 from all the identified proteins. The expression of these proteins in a baculovirus-insect cell expression system was successful only for Mb0309 and Mb3810. In parallel, we expressed the ESAT-6 family proteins EsxG and EsxH in this system. Among the recombinant proteins, Mb0309 and EsxG exhibited moderate performance in distinguishing between cattle that test positive and negative to bTB using the official test, the intradermal tuberculin test (IDT), when used to stimulate interferon-gamma production in blood samples from cattle. However, when combined as a protein cocktail, Mb0309 and EsxG were reactive in 50 % of positive cattle. Further assessments in cattle that evade the IDT (false negative) and cattle infected with Mycobacterium avium paratuberculosis are necessary to determine the potential utility of this cocktail as an additional tool to assist the accurate diagnosis of bTB.
العلاقة: https://doi.org/10.1016/j.vetimm.2024.110788Test; https://pubmed.ncbi.nlm.nih.gov/38838485Test
الإتاحة: https://doi.org/10.1016/j.vetimm.2024.110788Test
https://pubmed.ncbi.nlm.nih.gov/38838485Test -
2دورية أكاديمية
مصطلحات موضوعية: Baculovirus, Gfp, Polyhedrin, Sf9, Stable Cell Lines, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test
الوصف: Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign protein in lieu of the polyhedrin and hence do not form occlusion bodies (occ-), resulting in extremely reduced per os infectivity in larvae. To overcome this limitation, stably transformed insect cell lines expressing polyhedrin capable of occluding occ- recombinant baculovirus by trans-complementation were developed to obtain oral inoculum for insect larvae infection. First, the optimum regulatory region of polyhedrin promoter was determined utilizing chloramphenicol acetyl transferase (CAT) as the reporter gene. After infection with occ- baculovirus, the higher expression levels of CAT were achieved when a region of 2735 bp that contained sequences known to have transcriptional enhancer functions were present upstream the polyhedrin coding sequence. This regulatory region was selected to drive polyhedrin expression in insect cell lines. Transfection of Sf9 cells with plasmid carrying polyhedrin gene and stable cell lines established by selection with blasticidin showed polyhedrin expression and, moreover, crystalline polyhedron-like structures were visualized by optic microscopy. Oral infectivity was demonstrated by fluorescence detection in Rachiplusia nu larvae infected with occluded AcGFPpolh- baculovirus obtained using the system presented here. © 2009 Elsevier B.V. All rights reserved. ; Fil: López, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Carrillo, Elisa Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina ; Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; ...
وصف الملف: application/pdf
العلاقة: info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168165609004842Test; http://hdl.handle.net/11336/53645Test; López, María Gabriela; Alfonso, Victoria; Carrillo, Elisa Cristina; Taboga, Oscar Alberto; Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occ- baculovirus occlusion and larval per os infectivity; Elsevier Science; Journal of Biotechnology; 145; 2; 1-2010; 199-205; CONICET Digital; CONICET
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3دورية أكاديمية
المؤلفون: Molinari, Maria Paula, García Nuñez, Soledad, Gravisaco, María José, Carrillo, Elisa Cristina, Berinstein, Analía, Taboga, Oscar Alberto
مصطلحات موضوعية: BACULOVIRUS, FMDV, MICE, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test
الوصف: Foot-and-mouth disease virus (FMDV) causes a highly contagious and economically devastating disease that affects cattle, swine, goat and sheep among others. FMDV is able to overcome the initial host innate immune response by inhibiting the induction of antiviral molecules at both the transcriptional and the translational levels. It has been demonstrated that FMDV A/Arg/2001 causes the death of adult C57Bl/6 mice within 72. h. We evaluated the capacity of Autographa californica nuclear polyhedrosis virus (AcNPV), an insect virus with potent innate immunostimulating effects, to promote early protection against FMDV A/Arg/2001 challenge in C57Bl/6 mice. Groups of 8-9 weeks old female mice were injected intravenously with AcNPV and challenged with a lethal dose of FMDV at different times post-administration. Our results showed that pretreatment of mice with a single injection of AcNPV 3. h or 3 days before FMDV challenge resulted in complete abrogation of mortality and complete or partial suppression of viremia, respectively. Furthermore, no signs of disease were observed. AcNPV could be a valuable tool to improve the design of a novel vaccine that protects as an adjuvant at early times post-vaccination. ; Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: García Nuñez, Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentina ; Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de ...
وصف الملف: application/pdf
العلاقة: info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S016635421000611XTest; http://hdl.handle.net/11336/189972Test; Molinari, Maria Paula; García Nuñez, Soledad; Gravisaco, María José; Carrillo, Elisa Cristina; Berinstein, Analía; et al.; Baculovirus treatment fully protects mice against a lethal challenge of FMDV; Elsevier Science; Antiviral Research; 87; 2; 8-2010; 276-279; CONICET Digital; CONICET
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4دورية أكاديمية
المؤلفون: Costa Navarro, Guadalupe Soledad, Amalfi, Sabrina, López, María Gabriela, Llauger, Gabriela, Arneodo Larochette, Joel Demián, Taboga, Oscar Alberto, Alfonso, Victoria
مصطلحات موضوعية: AC12, ACMNPV, BACULOVIRUS, F-BOX, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test
الوصف: The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac12 gene, which is conserved in ten other baculovirus, codes a predicted 217 amino acid protein of unknown function. In this study, we investigated the role of ac12 during baculovirus infection, by generating an ac12 knockout virus. The transfection of the recombinant genome in insect cells resulted in unaltered viral dispersion and occlusion body production when compared to the control bacmid. This finding demonstrates that ac12 is a non-essential gene. Transmission and scanning electron microscopy (SEM) analyses showed that ac12 knockout virus produced occlusion bodies morphologically similar to those obtained with the control and capable to occlude virions. However, a slight but significant size difference was detected by SEM observation of purified occlusion bodies. This difference suggests that ac12 may be involved in regulatory pathways of polyhedrin production or occlusion body assembly without affecting either viral occlusion or oral infectivity in Rachiplusia nu larvae. This was evidenced by bioassays that showed no significant differences in the conditions tested. A qPCR analysis of viral gene expression during infection evidenced regulatory effects of ac12 over some representative genes of different stages of the viral cycle. In this study, we also showed that ac12 is transcribed at early times after infection and remains detectable up to 72 hours post-infection. The mRNA is translated during the infection and results in a protein that encodes an F-box domain that interacts in vivo and in vitro with S phase kinase associated protein 1 (SKP1) adaptor protein, which is potentially involved in protein ubiquitination pathways. ; Fil: Costa Navarro, Guadalupe Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque ...
وصف الملف: application/pdf
العلاقة: info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0168170218304775Test; http://hdl.handle.net/11336/147543Test; Costa Navarro, Guadalupe Soledad; Amalfi, Sabrina; López, María Gabriela; Llauger, Gabriela; Arneodo Larochette, Joel Demián; et al.; The autographa californica multiple nucleopolyhedrovirus Ac12: A non-essential F box-like protein that interacts with cellular SKP1 component of the E3 ubiquitin ligase complex; Elsevier Science; Virus Research; 260; 15-1-2019; 67-77; CONICET Digital; CONICET
