The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system
| العنوان: | The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system |
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| المؤلفون: | Pei, Z., Lang, B., Fragoso, Y.D., Shearer, K.D., Zhao, L., Mccaffery, P.J.A., Shen, S., Ding, Y.Q., McCaig, C.D., Collinson, J.M. |
| المصدر: | Neuroscience |
| بيانات النشر: | Elsevier Science, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | RT-PCR, reverse-transcription polymerase chain reaction, Male, Ndel1, rat hippocampus, Neuroscience(all), Ndel1, nuclear distribution protein nudE-like 1, PBS, phosphate-buffered saline, SVZ, subventricular zone, Cell Cycle Proteins, Hippocampus, Article, Cell Line, Prosencephalon, FBS, fetal bovine serum, Neural Stem Cells, nude, Lateral Ventricles, expression, Animals, Humans, HCNA94, Cells, Cultured, lis1, Neurons, neuronal migration, dynein, temporal-lobe epilepsy, SGZ, subgranular zone, Brain, differentiation, Middle Aged, Rats, schizophrenia, CNV, copy number variations, adult neurogenesis, Mice, Inbred C57BL, neurogenesis, cerebral cortical size, disc1, nervous system, Astrocytes, BSA, bovine serum albumin, Nde1, nuclear distribution factor E, Carrier Proteins, Nde1, Microtubule-Associated Proteins, psychological phenomena and processes, Disc1, disrupted in schizophrenia 1 |
| الوصف: | Graphical abstract Nde1 (red) is localised to the GFAP-positive (green) stem cell of the subventricular zone of the lateral ventricle, and its overexpression in neural stem cells promotes neuronal differentiation while inhibiting astroglial differentiation. Highlights • This is the most detailed morphological documentation of Nde1 and Ndel1 both in vivo and in vitro. • This is the first histological study of Nde1 expression in the healthy human cortex. • We demonstrate the localization of Nde1 and Ndel1 in neurogenic regions of the adult mouse brain. • Overexpression of Nde1 and Ndel1 leads to the fate specification changes of neural stem cells. Mental and neurological illnesses affect one in four people. While genetic linkage analyses have shown an association of nuclear distribution factor E (NDE1, or NudE) and its ohnolog NDE-like 1 (NDEL1, or Nudel) with mental disorders, the cellular mechanisms remain unclear. In the present study, we have demonstrated that Nde1 and Ndel1 are differentially localised in the subventricular zone (SVZ) of the forebrain and the subgranular zone (SGZ) of the hippocampus, two regions where neurogenesis actively occurs in the adult brain. Nde1, but not Ndel1, is localized to putative SVZ stem cells, and to actively dividing progenitors of the SGZ. The influence of these proteins on neural stem cell differentiation was investigated by overexpression in a hippocampal neural stem cell line, HCN-A94. Increasing Nde1 expression in this neural stem cell line led to increased neuronal differentiation while decreasing levels of astroglial differentiation. In primary cultured neurons and astrocytes, Nde1 and Ndel1 were found to have different but comparable subcellular localizations. In addition, we have shown for the first time that Nde1 is heterogeneously distributed in cortical astrocytes of human brains. Our data indicate that Nde1 and Ndel1 have distinct but overlapping distribution patterns in mouse brain and cultured nerve cells. They may function differently and therefore their dosage changes may contribute to some aspects of mental disorders. |
| اللغة: | English |
| تدمد: | 1873-7544 0306-4522 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::9afe7fa4edad2e7a4f35ad55e40da11fTest http://europepmc.org/articles/PMC4048543Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....9afe7fa4edad2e7a4f35ad55e40da11f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18737544 03064522 |
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