Immunoglobulin light chain (IgL) genes in zebrafish: Genomic configurations and inversional rearrangements between (VL–JL–CL) gene clusters
| العنوان: | Immunoglobulin light chain (IgL) genes in zebrafish: Genomic configurations and inversional rearrangements between (VL–JL–CL) gene clusters |
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| المؤلفون: | Gene Yeo, Lisa A. Steiner, Kerstin Howe, Anastasia M. Zimmerman, Benjamin J. Maddox |
| المصدر: | Developmental and Comparative Immunology |
| بيانات النشر: | Elsevier Science, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Immunoglobulin gene, Sequence analysis, Immunology, Molecular Sequence Data, Immunoglobulin Variable Region, Locus (genetics), Rearrangement, Immunoglobulin light chain, Genome, Article, Chromosomes, 03 medical and health sciences, 0302 clinical medicine, Immunoglobulin, Recombination signal sequences, Animals, Gene Rearrangement, B-Lymphocyte, Light Chain, Amino Acid Sequence, Zebrafish, Phylogeny, 030304 developmental biology, Genetics, 0303 health sciences, biology, Gene rearrangement, Sequence Analysis, DNA, biology.organism_classification, RSS, Multigene Family, Immunoglobulin Joining Region, Immunoglobulin Light Chains, Genes, Immunoglobulin Light Chain, Immunoglobulin Constant Regions, Sequence Alignment, 030215 immunology, Developmental Biology |
| الوصف: | In mammals, Immunoglobulin light chain (IgL) are localized to two chromosomal regions (designated kappa and lambda). Here we report a genome-wide survey of IgL genes in the zebrafish revealing (V(L)-J(L)-C(L)) clusters spanning 5 separate chromosomes. To elucidate IgL loci present in the zebrafish genome assembly (Zv6), conventional sequence similarity searches and a novel scanning approach based on recombination signal sequence (RSS) motifs were applied. RT-PCR with zebrafish cDNA was used to confirm annotations, evaluate VJ-rearrangement possibilities and show that each chromosomal locus is expressed. In contrast to other vertebrates in which IgL exon usage has been studied, inversional rearrangement between (V(L)-J(L)-C(L)) clusters were found. Inter-cluster rearrangements may convey a selective advantage for editing self-reactive receptors and poise zebrafish by virtue of their extensive numbers of V(L), J(L) and C(L) to have greater potential for immunoglobulin gene shuffling than traditionally studied mice and human models. |
| اللغة: | English |
| تدمد: | 1879-0089 0145-305X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed3aa30ecc0b4a2af68b00e636dc172cTest http://europepmc.org/articles/PMC3014032Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ed3aa30ecc0b4a2af68b00e636dc172c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18790089 0145305X |
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