دورية أكاديمية
Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity.
| العنوان: | Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity. |
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| المؤلفون: | Ripperger, Tyler J, Uhrlaub, Jennifer L, Watanabe, Makiko, Wong, Rachel, Castaneda, Yvonne, Pizzato, Hannah A, Thompson, Mallory R, Bradshaw, Christine, Weinkauf, Craig C, Bime, Christian, Erickson, Heidi L, Knox, Kenneth, Bixby, Billie, Parthasarathy, Sairam, Chaudhary, Sachin, Natt, Bhupinder, Cristan, Elaine, El Aini, Tammer, Rischard, Franz, Campion, Janet, Chopra, Madhav, Insel, Michael, Sam, Afshin, Knepler, James L, Capaldi, Andrew P, Spier, Catherine M, Dake, Michael D, Edwards, Taylor, Kaplan, Matthew E, Scott, Serena Jain, Hypes, Cameron, Mosier, Jarrod, Harris, David T, LaFleur, Bonnie J, Sprissler, Ryan, Nikolich-Žugich, Janko, Bhattacharya, Deepta |
| المصدر: | Immunity ; ISSN:1097-4180 ; Volume:53 ; Issue:5 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2020 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | COVID-19, S2 domain, SARS-CoV-2, antibodies, neutralization, nucleocapsid protein, orthogonal serological tests, receptor binding domain, serological test, serology, spike protein |
| الوصف: | We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies against the nucleocapsid (N) and the receptor binding domain (RBD) of the spike protein. Age and sex played lesser roles. All cases, including asymptomatic individuals, seroconverted by 2 weeks after PCR confirmation. Spike RBD and S2 and neutralizing antibodies remained detectable through 5-7 months after onset, whereas α-N titers diminished. Testing 5,882 members of the local community revealed only 1 sample with seroreactivity to both RBD and S2 that lacked neutralizing antibodies. This fidelity could not be achieved with either RBD or S2 alone. Thus, inclusion of multiple independent assays improved the accuracy of antibody tests in low-seroprevalence communities and revealed differences in antibody kinetics depending on the antigen. We conclude that neutralizing antibodies are stably produced for at least 5-7 months after SARS-CoV-2 infection. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.immuni.2020.10.004Test; https://pubmed.ncbi.nlm.nih.gov/33129373Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554472Test/ |
| DOI: | 10.1016/j.immuni.2020.10.004 |
| الإتاحة: | https://doi.org/10.1016/j.immuni.2020.10.004Test https://pubmed.ncbi.nlm.nih.gov/33129373Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554472Test/ |
| حقوق: | Copyright © 2020 Elsevier Inc. All rights reserved. |
| رقم الانضمام: | edsbas.E6ECC611 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.immuni.2020.10.004 |
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