Effect of structure in ionised albumin based nanoparticle: Characterisation, Emodin interaction, and in vitro cytotoxicity
| العنوان: | Effect of structure in ionised albumin based nanoparticle: Characterisation, Emodin interaction, and in vitro cytotoxicity |
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| المؤلفون: | Macarena Siri, Juan F. Delgado, Mariano Grasselli, Silvia del Valle Alonso, Jean-Marie Ruysschaert, Maria Julieta Fernandez Ruocco, Estefania Achilli, Malvina Pizzuto |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Materials Science and Engineering C, 103 |
| بيانات النشر: | Elsevier Science, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Physique de l'état condense [struct. électronique, etc.], DRUG DELIVERY, BOVINE SERUM ALBUMIN (BSA), Nanoparticle, 02 engineering and technology, Physique de l'état condense [struct. propr. thermiques, etc.], Microscopy, Atomic Force, 01 natural sciences, Technologie des autres industries, purl.org/becyt/ford/1 [https], Mice, chemistry.chemical_compound, Drug Delivery Systems, purl.org/becyt/ford/2.10 [https], Zeta potential, Bovine serum albumin, EMODIN, Physique de l'état condense [supraconducteur], Drug Carriers, biology, NF-kappa B, Serum Albumin, Bovine, Bioquímica y Biología Molecular, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, Chaotropic agent, Mechanics of Materials, Drug delivery, MCF-7 Cells, 0210 nano-technology, CIENCIAS NATURALES Y EXACTAS, Emodin, Materials science, Bioengineering, INGENIERÍAS Y TECNOLOGÍAS, Protein degradation, 010402 general chemistry, Fluorescence spectroscopy, Ciencias Biológicas, Biomaterials, Folic Acid, Microscopy, Electron, Transmission, Animals, Humans, purl.org/becyt/ford/1.6 [https], Nanotecnología, Macrophages, Tryptophan, Albumin, BSA-NANOPARTICLE (BSA NP), Nano-materiales, Biofísica, 0104 chemical sciences, Spectrometry, Fluorescence, Métallurgie et mines, Mécanique sectorielle, chemistry, purl.org/becyt/ford/2 [https], Gamma Rays, Ionic strength, biology.protein, Biophysics, Nanoparticles, Nuclear chemistry |
| الوصف: | A γ–irradiated bovine albumin serum-based nanoparticle was characterised structurally, and functionally. The nanoparticle was characterised by A.F.M. D.L.S, zeta potential, T.E.M. gel-electrophoresis, and spectroscopy. We studied the stability of the nanoparticle at different pH values and against time, by fluorescence spectroscopy following the changes in the tryptophan environment in the nanoparticle. The nanoparticle was also functionalized with Folic Acid, its function as a nanovehicle was evaluated through its interaction with the hydrophobic drug Emodin. The binding and kinetic properties of the obtained complex were evaluated by biophysical methods as well as its toxicity in tumor cells. According to its biophysics, the nanoparticle is a spherical nanosized vehicle with a hydrodynamic diameter of 70 nm. Data obtained describe the nanoparticle as nontoxic for cancer cell lines. When combined with Emodin, the nanoparticle proved to be more active on MCF-7 cancer cell lines than the nanoparticle without Emodin. Significantly, the albumin aggregate preserves the main activity-function of albumin and improved characteristics as an excellent carrier of molecules. More than carrier properties, the nanoparticle alone induced an immune response in macrophages which may be advantageous in vaccine and cancer therapy formulation. SCOPUS: ar.j info:eu-repo/semantics/published |
| وصف الملف: | application/pdf; 2 full-text file(s): application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e98c82a00b57c1a2f5cd0b6a2e9f1c94Test https://www.sciencedirect.com/science/article/abs/pii/S0928493119305466Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e98c82a00b57c1a2f5cd0b6a2e9f1c94 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |