The nanoscale organization of the B lymphocyte membrane☆
| العنوان: | The nanoscale organization of the B lymphocyte membrane☆ |
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| المؤلفون: | Palash Chandra Maity, Michael Reth, Kathrin Klaesener, Jianying Yang |
| المصدر: | Biochimica et Biophysica Acta |
| بيانات النشر: | Elsevier Pub. Co, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Nanocluster, Syk, spleen tyrosine kinase, Cell, Receptors, Cell Surface, Biology, Article, PALM, photoactivated localization microscopy, Cell membrane, dSTORM, direct stochastic optical reconstruction microscopy, Cell Compartmentation, medicine, Animals, Humans, TEM, transmission electron microscopy, Molecular Biology, B cell antigen receptor, Protein islands, B-Lymphocytes, PLA, proximity ligation assay, BN-PAGE, blue native polyacrylamide gel electrophoresis, Cell Membrane, breakpoint cluster region, Models, Immunological, Biological membrane, Cell Biology, BCR, B cell antigen receptor, Cell biology, medicine.anatomical_structure, Membrane, Membrane protein, PM, plasma membrane, Nanoparticles, Fluid mosaic model, TM, transmembrane |
| الوصف: | The fluid mosaic model of Singer and Nicolson correctly predicted that the plasma membrane (PM) forms a lipid bi-layer containing many integral trans-membrane proteins. This model also suggested that most of these proteins were randomly dispersed and freely diffusing moieties. Initially, this view of a dynamic and rather unorganized membrane was supported by early observations of the cell surfaces using the light microscope. However, recent studies on the PM below the diffraction limit of visible light (~ 250 nm) revealed that, at nanoscale dimensions, membranes are highly organized and compartmentalized structures. Lymphocytes are particularly useful to study this nanoscale membrane organization because they grow as single cells and are not permanently engaged in cell:cell contacts within a tissue that can influence membrane organization. In this review, we describe the methods that can be used to better study the protein:protein interaction and nanoscale organization of lymphocyte membrane proteins, with a focus on the B cell antigen receptor (BCR). Furthermore, we discuss the factors that may generate and maintain these membrane structures. Highlights • Fab-PLA, a new method to study principles of nanoscale organization of the PM • Nanoscale protein island organization of resting B cells alters upon activation • BCR dissociation and signal amplification involves Syk-mediated inside-out signaling |
| اللغة: | English |
| تدمد: | 0006-3002 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::436c2176277ef379c869f11a5ff32c87Test http://europepmc.org/articles/PMC4547082Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....436c2176277ef379c869f11a5ff32c87 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00063002 |
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