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Myosin light chain phosphorylation-dependent modulation of volume-regulated anion channels in macrovascular endothelium

التفاصيل البيبلوغرافية
العنوان: Myosin light chain phosphorylation-dependent modulation of volume-regulated anion channels in macrovascular endothelium
المؤلفون: Nilius, Bernd, Prenen, Jean, Walsh, M P, Carton, Iris, Bollen, Mathieu, Droogmans, Guillaume, Eggermont, Jan
بيانات النشر: Elsevier on behalf of the Federation of European Biochemical Societies
سنة النشر: 2000
المجموعة: KU Leuven: Lirias
مصطلحات موضوعية: Amino Acid Sequence, Animals, Anions, Cattle, Cells, Cultured, Endothelium, Vascular, Enzyme Inhibitors, Ion Channels, Molecular Sequence Data, Myosin Light Chains, Myosin-Light-Chain Kinase, Phosphorylation, Research Support, Non-U.S. Gov't
الوصف: The Rho/Rho-associated kinase (ROK) pathway has been shown to modulate volume-regulated anion channels (VRAC) in cultured calf pulmonary artery endothelial (CPAE) cells. Since Rho/ROK can increase myosin light chain phosphorylation, we have now studied the effects of inhibitors of myosin light chain kinase (MLCK) or myosin light chain phosphatase (MLCP) on VRAC in CPAE. Application of ML-9, an MLCK inhibitor, inhibited VRAC, both when applied extracellularly or when dialyzed into the cell. A similar inhibitory effect was obtained by dialyzing the cells with AV25, a specific MLCK inhibitory peptide. Conversely, NIPP1(191-210), an MLCP inhibitory peptide, potentiated the activation of VRAC by a 25% hypotonic stimulus. These data indicate that activation of VRAC is modulated by MLC phosphorylation. ; status: published
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 0014-5793
1873-3468
العلاقة: FEBS Letters vol:466 issue:2-3 pages:346-50; https://lirias.kuleuven.be/handle/123456789/9895Test; http://linkinghub.elsevier.com/retrieve/pii/S0014-5793Test(00)01097-8
الإتاحة: https://lirias.kuleuven.be/handle/123456789/9895Test
http://linkinghub.elsevier.com/retrieve/pii/S0014-5793Test(00)01097-8
رقم الانضمام: edsbas.1A4281A3
قاعدة البيانات: BASE
الوصف
تدمد:00145793
18733468