دورية أكاديمية
Additive enhancement of apoptosis by TRAIL and fenretinide in metastatic breast cancer cells in vitro
| العنوان: | Additive enhancement of apoptosis by TRAIL and fenretinide in metastatic breast cancer cells in vitro |
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| المؤلفون: | Ulukaya E., Sarimahmut M., Cevatemre B., Ari F., Yerlikaya A., Dimas K. |
| بيانات النشر: | Elsevier Masson SAS |
| سنة النشر: | 2014 |
| المجموعة: | Kütahya Dumlupınar University Institutional Repository |
| مصطلحات موضوعية: | Breast cancer, Chemotherapy, Cytotoxicity, Fenretinide, TRAIL |
| الوصف: | Successful management of metastatic breast cancer still needs better chemotherapeutic approaches. The combination of fenretinide (4-HPR), a synthetic retinoid inducing apoptosis by ROS generation, and TRAIL, a cell death ligand inducing caspase-dependent apoptosis, might result in more powerful cytotoxic activity. We therefore investigated the cytotoxic activity and resulting cell death mode of this combination in MDA-MB-231 cell line as a representative of metastatic state. Cytotoxicity was assessed by the ATP viability assay while the mode of cell death was determined both morphologically using fluorescence microscopy and biochemically using Western blotting and ELISA. The combination resulted in an additive cytotoxic effect at the doses used. Fragmented and/or pyknotic nuclei, which is a feature of apoptosis, were observed after treatment with fenretinide or TRAIL. However, the combinatorial treatment further increased apoptotic figures. Confirming apoptosis, active caspase-3 and cleaved PARP were increased by fenretinide or TRAIL in both western blotting and ELISA. Again, apoptosis was further increased by the combination. The combination warrants further studies due to its superior cytotoxic activity in the metastatic setting of breast cancer. © 2014 Elsevier Masson SAS. ; We appreciate the Research Fund of Uludag University for the financial support (the project number: UAP(T)-2010/37). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0753-3322 |
| العلاقة: | Biomedicine and Pharmacotherapy; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://dx.doi.org/10.1016/j.biopha.2014.03.013Test; https://hdl.handle.net/20.500.12438/4797Test; 68; 477; 482 |
| DOI: | 10.1016/j.biopha.2014.03.013 |
| الإتاحة: | https://doi.org/20.500.12438/4797Test https://doi.org/10.1016/j.biopha.2014.03.013Test https://hdl.handle.net/20.500.12438/4797Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.6422CBE8 |
| قاعدة البيانات: | BASE |
| تدمد: | 07533322 |
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| DOI: | 10.1016/j.biopha.2014.03.013 |