Many Families of C. elegans MicroRNAs Are Not Essential for Development or Viability
| العنوان: | Many Families of C. elegans MicroRNAs Are Not Essential for Development or Viability |
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| المؤلفون: | H. Robert Horvitz, Ezequiel Alvarez-Saavedra |
| المساهمون: | Massachusetts Institute of Technology. Department of Biology, Horvitz, H. Robert |
| المصدر: | PMC |
| بيانات النشر: | Elsevier Ltd. |
| مصطلحات موضوعية: | Mutant, Molecular Sequence Data, Oligonucleotides, Embryonic Development, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Article, DEV_BIO, microRNA, medicine, Morphogenesis, Animals, Caenorhabditis elegans, Oligonucleotide Array Sequence Analysis, Genetics, Regulation of gene expression, Mutation, biology, Agricultural and Biological Sciences(all), Base Sequence, Biochemistry, Genetics and Molecular Biology(all), RNA, Gene Expression Regulation, Developmental, biology.organism_classification, Phenotype, MicroRNAs, General Agricultural and Biological Sciences, Function (biology) |
| الوصف: | available in PMC 2010 August 23 MicroRNAs (miRNAs) are approximately 23 nt regulatory RNAs that posttranscriptionally inhibit the functions of protein-coding mRNAs. We previously found that most C. elegans miRNAs are individually not essential for development or viability and proposed that paralogous miRNAs might often function redundantly . To test this hypothesis, we generated mutant C. elegans strains that each lack multiple or all members of one of 15 miRNA families. Mutants for 12 of these families did not display strong synthetic abnormalities, suggesting that these miRNA families have subtle roles during development. By contrast, mutants deleted for all members of the mir-35 or mir-51 families died as embryos or early larvae, and mutants deleted for four members of the mir-58 family showed defects in locomotion, body size, and egg laying and an inability to form dauer larvae. Our findings indicate that the regulatory functions of most individual miRNAs and most individual families of miRNAs related in sequence are not critical for development or viability. Conversely, because in some cases miRNA family members act redundantly, our findings emphasize the importance of determining miRNA function in the absence of miRNAs related in sequence. Ellison Medical Foundation Howard Hughes Medical Institute |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0960-9822 |
| DOI: | 10.1016/j.cub.2009.12.051 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc8ebc1c0eb0ac1bb6b6ccde7b1e8252Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bc8ebc1c0eb0ac1bb6b6ccde7b1e8252 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09609822 |
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| DOI: | 10.1016/j.cub.2009.12.051 |