Proteomics towards the understanding of elicitor induced resistance of grapevine against downy mildew
| العنوان: | Proteomics towards the understanding of elicitor induced resistance of grapevine against downy mildew |
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| المؤلفون: | Christelle Lemaître-Guillier, Benoît Poinssot, Christine Schaeffer-Reiss, Sophie Trouvelot, Marielle Adrian, Agnès Hovasse, Xavier Daire, Marie-Claire Héloir, Ghislaine Recorbet |
| المساهمون: | Agroécologie [Dijon], Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), UMR 7178 Institut pluridisciplinaire Hubert Curien (IPHC) - Laboratoire de Spectrométrie de Masse BioOrganique, Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg ( UNISTRA ), BIVB (Bureau Interprofessionnel des Vins de Bourgogne), Conseil Regional de Bourgogne [2013 - 9201AA0050S02328], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Journal of Proteomics Journal of Proteomics, Elsevier, 2017, 156, pp.113-125. 〈10.1016/j.jprot.2017.01.016〉 Journal of Proteomics, Elsevier, 2017, 156, pp.113-125. ⟨10.1016/j.jprot.2017.01.016⟩ |
| بيانات النشر: | Elsevier BV, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Proteomics, 0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Biophysics, Laminarin, 01 natural sciences, Biochemistry, Plasmopara viticola, 03 medical and health sciences, Gene Expression Regulation, Plant, Botany, Plant defense against herbivory, Vitis, Leaf proteome, Glucans, Pathogen, Disease Resistance, Plant Diseases, Plant Proteins, 2. Zero hunger, Peronospora, [ SDV ] Life Sciences [q-bio], biology, food and beverages, Induced resistance, Biotic stress, 2-DE, biology.organism_classification, Elicitor, Plant Leaves, Fungicide, 030104 developmental biology, Vitis vinifera, Downy mildew, Electrophoresis, Polyacrylamide Gel, Resistance Process, 010606 plant biology & botany |
| الوصف: | Elicitors are known to trigger plant defenses in response to biotic stress, but do not systematically lead to effective resistance to pathogens. The reasons explaining such differences remain misunderstood. Therefore, elicitation and induced resistance (IR) were investigated through the comparison of two modified β-1,3 glucans applied on grapevine (Vitis vinifera) leaves before and after inoculation with Plasmopara viticola, the causal agent of downy mildew. The sulfated (PS3) and the shortened (H13) forms of laminarin are both known to elicit defense responses whereas only PS3 induces resistance against downy mildew. The analysis of the 2-DE gel electrophoresis revealed that PS3 and H13 induced distinct proteomic profiles after treatment and pathogen inoculation. Our results point out that the PS3-induced resistance is associated with the activation of the primary metabolism especially on amino acids and carbohydrates pathways. In addition, few proteins, such as the 12-oxophytodienoate reductase (OPR-like) related to the OPDA pathway, and an Arsenite-resistance protein (Serrate-like protein) could be considered as useful markers of induced resistance. Significance One strategy to reduce the application of fungicides is the use of elicitors which induce plant defense responses. Nonetheless, the elicitors do not systematically lead to resistance against pathogens. The lack of correlation between plant defense activation and induced resistance (IR) requires the investigation of what makes the specificity of elicitor-IR. In this study, the two β-glucans elicitors, sulfated (PS3) and short (H13) laminarins, were used in the grapevine/Plasmopara viticola interaction since only the first one leads to resistance against downy mildew. To disclose IR specificity, proteomic approach has been employed to compare the two treatments before and after P. viticola inoculation. The analysis of the 2-DE revealed that PS3 and H13 induced distinct proteomic profiles after treatment and pathogen inoculation. Significant increase of the number of proteins regulated by PS3, relative to both H13 and time-points, is correlated with the resistance process establishment. Our results point that the PS3-induced resistance requires the activation of the primary metabolism especially on amino acids and carbohydrates pathways. In addition, few proteins, such as the 12-oxophytodienoate reductase (OPR-like) related to the OPDA pathway, and an Arsenite-resistance protein (Serrate-like protein) could constitute useful markers of PS3 induced resistance. |
| تدمد: | 1874-3919 1876-7737 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb81f8c33c70ed7127d5d97943ccad33Test https://doi.org/10.1016/j.jprot.2017.01.016Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....eb81f8c33c70ed7127d5d97943ccad33 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18743919 18767737 |
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