A Chemogenetic Tool that Enables Functional Neural Circuit Analysis
| العنوان: | A Chemogenetic Tool that Enables Functional Neural Circuit Analysis |
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| المؤلفون: | Sharon Layfield, Ross A. D. Bathgate, Jaspreet K. Bassi, Mariana Rosso Melo, Andrew M. Allen, Hoai Buu Ngo, Stuart J. McDougall, Clément Menuet, Lin Xie, Angela A. Connelly |
| المساهمون: | Howard Florey Institute, University of Melbourne, Institut de Neurobiologie de la Méditerranée [Aix-Marseille Université] (INMED - INSERM U1249), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Grant funding was provided by the Australian NHMRC ( APP#1102477 and APP#1156727 ), the Australian Research Council ( DP170104582 ), and the State Government of Victoria Operational Infrastructure Support Program to the Florey Institute |
| المصدر: | Cell Reports Cell Reports, Elsevier Inc, In press, 32 (11), pp.108139. ⟨10.1016/j.celrep.2020.108139⟩ |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Nerve net, Blood Pressure, vagus, Rats, Sprague-Dawley, fluids and secretions, 0302 clinical medicine, Postsynaptic potential, Rats, Inbred SHR, polycyclic compounds, Homeostasis, Premovement neuronal activity, Transgenes, viral transduction, Neurons, Chemistry, viscerosensory system, virus diseases, Allatostatin, Vagus Nerve, allatostatin, nucleus of the solitary tract, medicine.anatomical_structure, Excitatory postsynaptic potential, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Rats, Transgenic, bodyweight, animal structures, Neuropeptide, Receptors, Cell Surface, CHO Cells, Inhibitory postsynaptic potential, digestive system, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Cricetulus, Solitary Nucleus, medicine, Animals, Humans, baroreceptor sensitivity, Amino Acid Sequence, Neurons, Afferent, Homeodomain Proteins, Body Weight, Neuropeptides, digestive system diseases, Electrophysiological Phenomena, HEK293 Cells, 030104 developmental biology, nervous system, Synapses, chemogenetics, Neuron, Nerve Net, Neuroscience, 030217 neurology & neurosurgery |
| الوصف: | Chemogenetics enables manipulation of neuronal activity in experimental animals. While providing information about the transduced neuron expressing a ligand-activated molecule, chemogenetics does not provide understanding about the antecedent circuit that drives that neuron's activity. For current approaches, this is not feasible, because the activating molecules are not genetically encoded. The insect allatostatin/allatostatin receptor system, a highly specific, powerful inhibitory chemogenetic approach, has this advantage, because the ligand, being a peptide, is genetically encoded. We developed viral vector-based systems to express biologically active allatostatin in neurons in vivo and allatostatin receptors in subpopulations of postsynaptic neurons. We demonstrate that activity-dependent release of allatostatin induces inhibition of allatostatin receptor-expressing neurons. We validate the approach in the vagal viscerosensory system where inhibitory, rather than the usual excitatory, viscerosensory input leads to sustained decreases in baroreceptor reflex sensitivity and bodyweight. |
| تدمد: | 2211-1247 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a93fded8cb2971750b7661522e765feTest https://doi.org/10.1016/j.celrep.2020.108139Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8a93fded8cb2971750b7661522e765fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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