Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance
| العنوان: | Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance |
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| المؤلفون: | Carlos Vilela, Le-Ping Yan, Joaquim M. Oliveira, Joana Silva-Correia, Ana Oliveira, Hélder Pereira, Mariana B. Oliveira, Rui A. Sousa, Rui L. Reis, João F. Mano |
| المساهمون: | Universidade do Minho, Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| المصدر: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| بيانات النشر: | Elsevier BV, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Calcium Phosphates, Scaffold, Materials science, Silk, Biomedical Engineering, Silk fibroin, Fibroin, macromolecular substances, In Vitro Techniques, Osteochondral regeneration, Biochemistry, Bone and Bones, Biomaterials, Tissue engineering, In vivo, medicine, Animals, Bilayered scaffold, Molecular Biology, Science & Technology, Nanocomposite, Tissue Engineering, Tissue Scaffolds, Regeneration (biology), Cartilage, fungi, Mesenchymal stem cell, technology, industry, and agriculture, X-Ray Microtomography, General Medicine, Adhesion, equipment and supplies, medicine.anatomical_structure, SILK, Calcium phosphate, Rabbits, Biotechnology, Biomedical engineering |
| الوصف: | Novel porous bilayered scaffolds, fully integrating a silk fibroin (SF) layer and a silk-nano calcium phosphate (Silk-NanoCaP) layer for osteochondral defect (OCD) regeneration were developed. Homogeneous porosity distribution was achieved in the scaffolds, with calcium phosphate phase only retained in the Silk-NanoCaP layer. The scaffold presented compressive moduli of 0.4 MPa in wet state. Rabbit bone marrow mesenchymal stromal cells (RBMSCs) were cultured on the scaffolds, and good adhesion and proliferation were observed. The Silk-NanoCaP layer showed a higher alkaline phosphatase level than the silk layer in osteogenic conditions. Subcutaneous implantation in rabbits demonstrated weak inflammation. In a rabbit knee critical size OCD model, the scaffolds firmly integrated into the host tissue. The histological analysis and immunohistochemistry analysis showed that collagen II positive cartilage and glycosaminoglycan regeneration presented in the silk layer, and de novo bone ingrowths and vessel formation were observed in the Silk-NanoCaP layer. These bilayered scaffolds can therefore be promising candidates for OCD regeneration. This study was funded by the Portuguese Foundation for Science and Technology (FCT) projects Tissue2Tissue (PTDC/CTM/105703/2008) and OsteoCart (PTDC/CTM-BPC/115977/2009), as well as the European Union's FP7 Programme under grant agreement no REGPOT-CT2012-316331-POLARIS. The authors are grateful to Viviana P. Ribeiro and Teresa Oliveira for the assistance with the immunohistochemical staining. L-P.Y. was awarded a FCT PhD scholarship (SFRH/BD/64717/2009). The FCT distinction attributed to J.M.O. under the Investigator FCT program (IF/00423/2012) is also greatly acknowledged. |
| وصف الملف: | application/pdf |
| تدمد: | 1742-7061 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46082a32ffc402f41dc41a0a21cd1073Test https://doi.org/10.1016/j.actbio.2014.10.021Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....46082a32ffc402f41dc41a0a21cd1073 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17427061 |
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