Maternally-derived neutralizing antibodies reduce vaccine efficacy against porcine reproductive and respiratory syndrome virus infection
| العنوان: | Maternally-derived neutralizing antibodies reduce vaccine efficacy against porcine reproductive and respiratory syndrome virus infection |
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| المؤلفون: | Mathieu Andraud, Valérie Normand, Olivier Bourry, Patricia Renson, Frédéric Paboeuf, Christelle Fablet, Arnaud Lebret, Nicolas Rose |
| المصدر: | Vaccine |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | PRRS virus, Swine, animal diseases, Antibodies, Viral, IFNa, fluids and secretions, Immunogenicity, Vaccine, 0302 clinical medicine, 030212 general & internal medicine, Neutralizing antibody, integumentary system, biology, Vaccination, virus diseases, respiratory system, Infectious Diseases, Molecular Medicine, Antibody, 030231 tropical medicine, Porcine Reproductive and Respiratory Syndrome, Modified live virus vaccine, Viremia, Vaccines, Attenuated, Article, Virus, 03 medical and health sciences, Neutralization Tests, medicine, Animals, Porcine respiratory and reproductive syndrome virus, Immunization Schedule, General Veterinary, General Immunology and Microbiology, Inoculation, Maternally derived antibody, Public Health, Environmental and Occupational Health, Interferon-alpha, Viral Vaccines, Vaccine efficacy, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, medicine.disease, Antibodies, Neutralizing, Virology, biology.protein, Interference, Immunity, Maternally-Acquired, Biomarkers |
| الوصف: | Highlights • Maternally-derived neutralizing antibodies impair PRRS vaccination immune responses in piglets. • Maternally-derived neutralizing antibodies reduce PRRS vaccine efficacy in piglets. • IFNa may also interfere with PRRS vaccination. Modified live virus (MLV) vaccines are commonly used to reduce the impact of porcine reproductive and respiratory syndrome (PRRS) but limited efficacy is achieved in field conditions. Here, we evaluated the impact of maternally-derived neutralizing antibodies (MDNAs) on vaccine efficacy after PRRS virus (PRRSV) challenge. Piglets with low (A−) or high (A+) MDNA levels derived from a commercial pig herd were moved to experimental facilities to be vaccinated (V+) or not (V−) with a PRRSV-1 MLV vaccine at 3 weeks of age (woa). Because of unexpectedly low vaccine detection in A−V+ piglets post-vaccination (pv), all V+ piglets received a second vaccination at 4 woa. Five weeks (W5) pv, piglets were inoculated with a PRRSV-1 field strain to evaluate vaccine protection, and were mingled 24 h later with non-inoculated piglets of similar immune status to assess viral transmission. Vaccine strain was detected at W2 pv in 69% and 6% of A−V+ and A+V+ piglets, and at W5 pv in 50% and 25% of A−V+ and A+V+ piglets, respectively. At W5 pv, 94% of A−V+ and 44% of A+V+ piglets seroconverted, with a significant IFNg response induction in the A−V+ group only. After challenge, compared to the V− inoculated group, viremia was 100-fold lower at 10 days post-infection in A−V+ whereas viremia was not significantly reduced in A+V+ piglets. A lower transmission rate was estimated for the A−V+ group: 0.15 [0.07–0.29] versus 0.44 [0.18–1.76] and 0.32 [0.14–0.68] for the A+V+ and V− groups, respectively. Investigations about the low vaccine strain detection after the first vaccination suggested a relationship between IFNa levels and vaccine strain detection in A−V+ piglets. We showed that MDNAs impair vaccine efficacy against PRRSV both in inoculated and contact piglets, probably by reducing vaccine replication. IFNa may also interfere with PRRSV vaccination. These new data could help improving vaccination protocols. |
| تدمد: | 0264-410X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c09e3333707e1cfa9a353718667b3eTest https://doi.org/10.1016/j.vaccine.2019.06.045Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f2c09e3333707e1cfa9a353718667b3e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0264410X |
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