Automated Electron Tomography of the Septal Pore Cap in Rhizoctonia solani
| العنوان: | Automated Electron Tomography of the Septal Pore Cap in Rhizoctonia solani |
|---|---|
| المؤلفون: | Bruno M. Humbel, Wally H. Müller, Ulrike Ziese, Teun Boekhout, Roy Christiaan Montijn, Abraham J. Koster, Arie J. Verkleij, A.C. van Aelst, T.P. van der Krift |
| المساهمون: | Centraal Instituut voor Voedingsonderzoek TNO |
| المصدر: | Journal of Structural Biology, 1, 131, 10-18 Journal of Structural Biology 131 (2000) Journal of Structural Biology, 131, 10-18 |
| بيانات النشر: | Elsevier BV, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Laboratorium voor Plantencelbiologie, Endoplasmic Reticulum, Dolipore septum, Cryo-scanning electron microscopy, Cell membrane, Automation, Structural Biology, Freezing, Image Processing, Computer-Assisted, Tomography, Diagnostic value, Priority journal, Fungus, High-pressure freezing, Vesicle, Cell ultrastructure, Mitochondria, Cell biology, Laboratory of Plant Cell Biology, Membrane structure, Intercellular Junctions, medicine.anatomical_structure, 6-glucan, Electron beam tomography, Channel gating, Filamentous structures, Thanatephorus cucumeris, animal structures, β-1,6-glucan, Electrons, Biology, Septal pore cap, Cell communication, Rhizoctonia, ZIO staining, Organelle, medicine, β-1, Nutrition, Hyphomycetes, Rhizoctonia solani, Basidiomycota, Endoplasmic reticulum, Cell Membrane, Cryoelectron Microscopy, fungi, Botany, Fungi, Intracellular Membranes, Nonhuman, Microscopy, Electron, Electron tomography, Freeze substitution, Microscopy, Electron, Scanning, Ultrastructure, Transmission electron microscopy |
| الوصف: | Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hypal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the SPC with associated cell ultrastructures in three dimensions by automated electron tomography of thick-sectioned cells, followed by 3D tomographic reconstructions. Using these methods we were able to document the passage of mitochondria through the SPC, small tubular membranous structures at the entrance of the septal pore channel, filamentous structures connecting the inner side of the SPC with pore-plugging material, thin filaments anchoring the pore-plugging material with the plasma membrane, small vesicles attached to the plugging material, and tubular endoplasmic reticulum continuous with the base of the SPC. We hypothesize that the SPC, the filamentous structures, the plugging material, and the endoplasmic reticulum act in a coordinated fashion to maintain cellular integrity, intercellular communication, and the transport of solutes and cell organelles in the filamentous fungus R. solani. (C) 2000 Academic Press. |
| وصف الملف: | application/pdf; application/octet-stream |
| تدمد: | 1047-8477 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4eaf523659c30cfb859c4eaa59c1afc0Test https://doi.org/10.1006/jsbi.2000.4243Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4eaf523659c30cfb859c4eaa59c1afc0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10478477 |
|---|