Preparation of gellan-cholesterol nanohydrogels embedding baicalin and evaluation of their wound healing activity
| العنوان: | Preparation of gellan-cholesterol nanohydrogels embedding baicalin and evaluation of their wound healing activity |
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| المؤلفون: | Nicole Zoratto, Anna Maria Fadda, Maria Letizia Manca, Amparo Nácher, Carla Caddeo, Claudia Cencetti, Maria Manconi, Pietro Matricardi, Chiara Di Meo |
| المصدر: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Biocompatibility, Swine, Sonication, Wound healing, Pharmaceutical Science, Biocompatible Materials, 02 engineering and technology, antioxidant activity, baicalin, fibroblasts, gellan, nanohydrogel, wound healing, biotechnology, 3003, 01 natural sciences, Cell Line, Mice, chemistry.chemical_compound, Antioxidant activity, In vivo, 0103 physical sciences, Animals, Baicalin, Hydrogen peroxide, Skin, Flavonoids, Drug Carriers, Wound Healing, 010304 chemical physics, biology, Polysaccharides, Bacterial, Hydrogels, 3T3 Cells, General Medicine, Fibroblasts, 021001 nanoscience & nanotechnology, In vitro, Nanostructures, Cholesterol, chemistry, Gellan, Myeloperoxidase, biology.protein, Biophysics, Nanoparticles, Female, Nanohydrogel, 0210 nano-technology, Biotechnology |
| الوصف: | [EN] In the present work, the preparation, characterization and therapeutic potential of baicalin-loaded nanohydrogels are reported. The nanohydrogels were prepared by sonicating (S nanohydrogel) or autoclaving (A nanohydrogel) a dispersion of cholesterol-derivatized gellan in phosphate buffer. The nanohydrogel obtained by autoclave treatment showed the most promising results: smaller particles ( similar to 362 nm vs. similar to 530 nm), higher homogeneity (polydispersity index = similar to 0.24 vs. similar to 0.47), and lower viscosity than those obtained by sonication. In vitro studies demonstrated the ability of the nanohydrogels to favour the deposition of baicalin in the epidermis. A high biocompatibility was found for baicalin-loaded nanohydrogels, along with a great ability to counteract the toxic effect induced by hydrogen peroxide in cells, as the nanohydrogels re-established the normal conditions (similar to 100% viability). Further, the potential of baicalin-loaded nanohydrogels in skin wound healing was demonstrated in vivo in mice by complete skin restoration and inhibition of specific inflammatory markers (i.e., myeloperoxidase, tumor necrosis factor-alpha, and oedema. Financial support from University "Sapienza" - Progetti di Ricerca: grant RP116154C2EF9AC8 and grant RM11715C1743EE89 are acknowledged. |
| وصف الملف: | application/pdf |
| تدمد: | 0939-6411 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec5a7b2c02490db99b4276113c0f4527Test https://doi.org/10.1016/j.ejpb.2018.02.015Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ec5a7b2c02490db99b4276113c0f4527 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09396411 |
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