Human aquaporin-11 guarantees efficient transport of H2O2 across the endoplasmic reticulum membrane
| العنوان: | Human aquaporin-11 guarantees efficient transport of H2O2 across the endoplasmic reticulum membrane |
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| المؤلفون: | Roberto Sitia, Paolo Pinton, Stefano Bestetti, Alessandro Rimessi, Iria Medraño-Fernandez, Mauro Galli, Ilaria Sorrentino |
| المساهمون: | Bestetti, S., Galli, M., Sorrentino, I., Pinton, P., Rimessi, A., Sitia, R., Medrano-Fernandez, I. |
| المصدر: | Redox Biology Redox Biology, Vol 28, Iss, Pp-(2020) |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Clinical Biochemistry, MAM, mitochondria-associated ER membranes, Endoplasmic Reticulum, Biochemistry, 0302 clinical medicine, Chlorocebus aethiops, Peroxiporins, lcsh:QH301-705.5, lcsh:R5-920, Chemistry, Aquaporin, Cell biology, Aquaporins, Peroxiporins, Hydrogen peroxide, Membrane permeability, Endoplasmic reticulum, Redox homeostasis, Membrane permeability, Membrane, H2O2, hydrogen peroxide, COS Cells, lcsh:Medicine (General), Redox homeostasis, Intracellular, Research Paper, Socio-culturale, Down-Regulation, CHO Cells, Aquaporins, ER, endoplasmic reticulum, 03 medical and health sciences, Cricetulus, Downregulation and upregulation, Peroxiporin, Animals, Humans, AQP, aquaporins, Endoplasmic reticulum, Cell Membrane, Organic Chemistry, Biological Transport, Hydrogen Peroxide, Hydrogen peroxide, Cytosol, HEK293 Cells, 030104 developmental biology, lcsh:Biology (General), DTT, dithiothreitol, Flux (metabolism), 030217 neurology & neurosurgery, HeLa Cells |
| الوصف: | Hydrogen peroxide (H2O2) is an essential second intracellular messenger. To reach its targets in the cytosol, H2O2 must cross a membrane, a feat that requires aquaporins (AQP) endowed with ‘peroxiporin’ activity (AQP3, AQP8, AQP9). Here, we exploit different organelle-targeted H2O2-sensitive probes to show that also AQP11 efficiently conduits H2O2. Unlike other peroxiporins, AQP11 is localized in the endoplasmic reticulum (ER), accumulating partly in mitochondrial-associated ER membranes (MAM). Its downregulation severely perturbs the flux of H2O2 through the ER, but not through the mitochondrial or plasma membranes. These properties make AQP11 a potential regulator of ER redox homeostasis and signaling. Graphical abstract Image 1 Highlights • AQP11 is an endoplasmic reticulum (ER)-resident peroxiporin. • AQP11 allows H2O2 fluxes across the ER membrane. • Its levels impact ER redox homeostasis. |
| تدمد: | 2213-2317 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a45d5088584369da32632bc30d11561dTest https://doi.org/10.1016/j.redox.2019.101326Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a45d5088584369da32632bc30d11561d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22132317 |
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