التفاصيل البيبلوغرافية
| العنوان: |
Development of a colorimetric and fluorescent Cu2+ ion probe based on 2′-hydroxy-2,4-diaminoazobenzene and its application in real water sample and living cells. |
| المؤلفون: |
Hong, Ren1 (AUTHOR) renhong_jl@163.com, Ping, Wu1 (AUTHOR), Fei, Li1 (AUTHOR), Shihua, Yu1 (AUTHOR), Li, Jin1 (AUTHOR), Dawei, Lou1 (AUTHOR) |
| المصدر: |
Inorganica Chimica Acta. Jul2020, Vol. 507, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*FLUORESCENCE yield, *WATER sampling, *POTASSIUM ions, *ETHYLENEDIAMINE, *SILVER ions, *BENZOTRIAZOLE derivatives, *AMINO group |
| مستخلص: |
• L1-OH coordinated with Cu2+ to form 1:1 complex. • L1-OH was catalyzed oxidative cyclization by Cu2+ to fluorescent benzotriazole. • L1-OH can detect Cu2+ ion in water sample with apparent color change. • The fluorescence imaging of living cells HepG2 shows the application of L1-OH. 2′-Hydroxy-2,4-diaminoazobenzene (L1-OH), a fluorescence probe bearing ortho hydroxyl and amino group for the detection of Cu2+ ion was synthesized through diazo coupling reaction. The visible color change of L1-OH from yellow to pink was attributed to the coordination with Cu2+. The 1:1 binding mode of L1-OH with Cu2+ was confirmed by UV titration, Job-plot method and ethylene diamine tetraacetic acid (EDTA) chelation experiment. Meanwhile, the non-emissive L1-OH (Fluorescence quantum yield, Φ = 0.003) has transformed into fluorescent benzotriazole derivative 2-(2-hydroxy-phenyl)–2H-5-aminobenzo[d][1–3]triazole (L1-OH-BTA) (Φ = 0.27) by adding Cu2+, which was supported by the HPLC spectra and DFT calculations. L1-OH was applied for quantitative detection of Cu2+ with good linear relationship between UV absorbance, fluorescence intensity and Cu2+ concentration, and exhibited high selectivity toward Cu2+ over other metal ions (Na+, K+, Ca2+, Ag+, Pb2+, Zn2+, Mn2+, Mg2+, Ni2+, Fe2+, Fe3+, Co2+, Cd2+ and Al3+). Furthermore, the test paper dyed by L1-OH shown colorimetric detection soaking in practical water sample with Cu2+ ion (≥10 μM). The L1-OH could also be used as a fluorescent probe for monitoring Cu2+ ion in living HepG2 cells. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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