Long-term amphetamine treatment exacerbates inflammatory lung reaction while decreases airway hyper-responsiveness after allergic stimulus in rats
| العنوان: | Long-term amphetamine treatment exacerbates inflammatory lung reaction while decreases airway hyper-responsiveness after allergic stimulus in rats |
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| المؤلفون: | Isabel Daufenback Machado, Sandra Helena Poliselli Farsky, Amílcar Sabino Damazo, Adriana Lino-dos-Santos-Franco, E.K. Hamasato, Wothan Tavares-de-Lima, Rodolfo de Paula Vieira, João Antônio Gimenes Júnior, João Palermo-Neto, Beatriz Golegã Acceturi, Ana Paula Ligeiro de Oliveira |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Elsevier B.V. |
| مصطلحات موضوعية: | Male, Ovalbumin, medicine.medical_treatment, Immunology, Inflammation, Pharmacology, Drug Administration Schedule, Drug Hypersensitivity, RATOS, Adhesion molecules expression, medicine, Animals, Immunology and Allergy, Rats, Wistar, Sympathomimetics, Amphetamine, Sensitization, Airways reactivity, biology, medicine.diagnostic_test, Bronchial Spasm, business.industry, Degranulation, respiratory system, Asthma, Rats, medicine.anatomical_structure, Cytokine, Bronchoalveolar lavage, Gene Expression Regulation, biology.protein, Cytokines, Rat, Methacholine, medicine.symptom, business, Bronchoalveolar Lavage Fluid, medicine.drug |
| الوصف: | article i nfo Asthma is an allergic lung disease can be modulated by drugs that modify the activity of central nervous sys- tem (CNS) such as amphetamine (AMPH). AMPH is a highly abused drug that exerts potent effects on behav- ior and immunity. In this study we investigated the mechanism involved in the effects of long-term AMPH treatment on the increased magnitude of allergic lung response. We evaluated mast cells degranulation, cy- tokines release, airways responsiveness and, expression of adhesion molecules. Male Wistar rats were treated with AMPH or vehicle (PBS) for 21 days and sensitized with ovalbumin (OVA) one week after the first injec- tion of vehicle or AMPH. Fourteen days after the sensitization, the rats were challenged with an OVA aerosol, and 24 h later their parameters were analyzed. In allergic rats, the treatment with AMPH exacerbated the lung cell recruitment due increased expression of ICAM-1, PECAM-1 and Mac-1 in granulocytes and macro- phages recovered from bronchoalveolar lavage. Elevated levels of IL-4, but decreased levels of IL-10 were also found in samples of lung explants after AMPH treatment. Conversely, the ex-vivo tracheal hyper-responsiveness to methacholine (MCh) was reduced by AMPH treatment, whereas the force contrac- tion of tracheal segments due to in vitro antigen challenge remained unaltered. Our findings suggest that lung inflammation and airway hyper-responsiveness due to OVA challenge are under the distinct control of AMPH during long-term treatment. Our data strongly indicate that AMPH pos- itively modulates allergic lung inflammation via the increase of ICAM-1, PECAM-1, Mac-1 and IL-4. AMPH also abrogates the release of the anti-inflammatory cytokine IL-10. |
| اللغة: | English |
| تدمد: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2012.09.009 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23a671cdfef54acae528b0848aef3aa3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....23a671cdfef54acae528b0848aef3aa3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15675769 |
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| DOI: | 10.1016/j.intimp.2012.09.009 |