التفاصيل البيبلوغرافية
| العنوان: |
LPA19, a Psb27 Homolog in Arabidopsis thaliana, Facilitates D1 Protein Precursor Processing during PSII Biogenesis. |
| المؤلفون: |
Lili Wei1, Jinkui Guo1, Min Ouyang1, Xuwu Sun1, Jinfang Ma1, Wei Chi1, Congming Lu1, Tixin Zhang1 zhanglixin@ibcas.ac.cn |
| المصدر: |
Journal of Biological Chemistry. 7/9/2010, Vol. 285 Issue 28, p21391-21398. 8p. 1 Color Photograph, 1 Diagram, 1 Chart, 6 Graphs. |
| مصطلحات موضوعية: |
*ARABIDOPSIS thaliana, *PROTEINS, *ORIGIN of life, *GENETIC mutation, *CHLOROPLASTS |
| مستخلص: |
The biogenesis and assembly of photosystem II (PSII) are mainly regulated by the nuclear-encoded factors. To further identify the novel components involved in PSII biogenesis, we isolated and characterized a high chlorophyll fluorescence low psii accumulationl9 (lpa19) mutant, which is defective in PSII biogenesis. LPA19 encodes a Psb27 homolog (Atlg05385). Interestingly, another Psb27 homolog (Atlg03600) in Arabidopsis was revealed to be required for the efficient repair of photodamaged PSII. These results suggest that the Psb27 homologs play distinct functions in PSII biogenesis and repair in Arabidopsis. Chloroplast protein labeling assays showed that the C-terminal processing of D1 in the lpa19 mutant was impaired. Protein overlay assays provided evidence that LPA19 interacts with D1, and coimmunoprecipitation analysis demonstrated that LPA19 interacts with mature D1 (roD1) and precursor D1 (pD1). Moreover, LPA19 protein was shown to specifically interact with the soluble C terminus present in the precursor and mature D1 through yeast two-hybrid analyses. Thus, these studies suggest that LPA 19 is involved in facilitating the D1 precursor protein processing in Arabidopsis. [ABSTRACT FROM AUTHOR] |
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