Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate
| العنوان: | Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate |
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| المؤلفون: | Liu Xu, Liu Cui, Fan Yunpeng, Hu YuanLiang, Wang De-yun, Sun Zhan’ao, Yi Fang, Zhang Baokang, Liu Dan, Liu Jiaguo, Zhang Yu-qing |
| المصدر: | International Journal of Biological Macromolecules |
| بيانات النشر: | Elsevier B.V., 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, WDFF-BSRF-SF, wild dendranthema flower flavone plus baical skullcap root flavone plus sanchi flavone, Chick Embryo, Pharmacology, Antibodies, Viral, Biochemistry, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Flavone ingredients prescriptions, WDFF-BSRF, wild dendranthema flower flavone plus baical skullcap root flavone, Structural Biology, CHMIs, Chinese herb medicinal ingredients, EF, epimedium flavone, BSRF, baical skullcap root flavone, Infectivity, chemistry.chemical_classification, Epimedium, EF-WDFF-BSRF, epimedium flavone plus wild dendranthema flower flavone plus baical skullcap root flavone, Antibody titer, ND, Newcastle disease, General Medicine, EF-BSRF, epimedium flavone plus baical skullcap root flavone, Protective rate, Titer, CHMs, Chinese herbal medicines, Antibody, HI, hemagglutination inhibition, medicine.drug_class, Newcastle Disease, WDFF, wild dendranthema flower flavone, PBS, phosphate-buffered saline, Newcastle disease virus, CMF, calcium and magnesium-free, Biology, EF-WDFF, epimedium flavone plus wild dendranthema flower flavone, BC, blank control, Flavones, Antiviral Agents, Virus, Article, MEM, minimum essential medium, Antivirus, medicine, SF, sanchi flavone, Animals, Mortality, Molecular Biology, Highest virus inhibitory rate, CEF, chicken embryo fibroblast, EF-WDFF-SF, epimedium flavone plus wild dendranthema flower flavone plus sanchi flavone, EF-BSRF-SF, epimedium flavone plus baical skullcap root flavone plus sanchi flavone, Plant Extracts, EF-SF-WDFF-BSRF, epimedium flavone plus sanchi flavone plus wild dendranthema flower flavone plus baical skullcap root flavone, DMSO, Dimethyl sulfoxide, Viral Vaccines, biology.organism_classification, MM, maintenance medium, EF-SF, epimedium flavone plus sanchi flavone, Virology, chemistry, NDV, Newcastle disease virus, VC, vaccine control, biology.protein, Antiviral drug, Morbidity, Chickens |
| الوصف: | Highlights ► Flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect. ► EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF could significantly inhibit the cellular infectivity of NDV in CEF. ► EF-SF-WDFF-BSRF could improve the protective effect of ND vaccine. ► EF-SF-WDFF-BSRF would be expected to exploit into a new-type antiviral drug. In order to screen better flavone prescriptions of anti-Newcastle disease virus (NDV), four flavone ingredients of epimedium flavones (EF), baikal skullcap root flavones (BSRF), wild dendranthema flower flavones (WDFF), and sanchi flavones (SF) screened in previous experiments and their prescriptions were added into chicken embryo fibroblast monolayer with three drug-adding modes respectively. The cellular A570 values, the highest virus inhibitory rates and the score based on virus inhibitory rate were calculated to compare their antiviral activity. In immune protective test, the effects of three preparations (EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF screened by the results in vitro experiment) on NDV infection were compared in chickens vaccinated with ND vaccine then challenged with NDV. Blood was regularly sampled for serum antibody titer determination. The pathogenic and dead statuses of chickens were clinically examined. The results indicated that the A570 values of the nine prescriptions, especially the foresaid three prescriptions at almost all concentrations in three drug-adding modes were significantly higher than that of the virus control group. The foresaid three prescriptions presented at the top five of the highest virus inhibitory rate, and located at the highest three of the score rank. The antibody titers and protective rates of the three prescriptions groups were higher than that of VC group, especially EF-SF-WDFF-BSRF group showed significant difference. These results indicated that flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect, ES-SF-WDFF-BSRF prescription could inhibit the cellular infectivity of NDV, improve the protective effect of ND vaccine and would be expected to exploit into a new-type antiviral drug. |
| اللغة: | English |
| تدمد: | 1879-0003 0141-8130 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e356b1423930e9045616a8e070e4cc36Test http://europepmc.org/articles/PMC7124392Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e356b1423930e9045616a8e070e4cc36 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18790003 01418130 |
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