Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene
| العنوان: | Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene |
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| المؤلفون: | De-nian Miao, Wan-guo Su, Yong Zou, Jianguo Zhu, Xiuguo Hua, Junfang Zhou, Xi-zhong He, Li Cui |
| المصدر: | Antiviral Research |
| بيانات النشر: | Elsevier B.V., 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | RNA interference (RNAi), RdRp, Genes, Viral, Swine, RNA-dependent RNA polymerase, medicine.disease_cause, Transfection, Virus Replication, Sensitivity and Specificity, Virus, Article, Cell Line, Small hairpin RNA, chemistry.chemical_compound, RNA interference, TGEV, Virology, RNA polymerase, medicine, Coronaviridae, Animals, Cytopathic effect, Coronavirus, Pharmacology, biology, Gastroenteritis, Transmissible, of Swine, Transmissible gastroenteritis virus, biology.organism_classification, RNA-Dependent RNA Polymerase, ST, chemistry, Short hairpin RNA (shRNA), RNA Interference, Plasmids |
| الوصف: | Transmissible gastroenteritis virus (TGEV) is identified as one of the most important pathogenic agents during swine enteric infection, leading to high mortality in neonatal pigs and severe annual economic loss in swine-producing areas. Up to date, various vaccines developed against TGEV still need to be improved. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, two shRNA-expressing plasmids (pEGFP-U6/P1 and pEGFP-U6/P2) targeting the RNA-dependent RNA polymerase (RdRp) gene of TGEV were constructed and transfected into swine testicular (ST) cells. The cytopathic effect (CPE) and MTS assays demonstrated that both shRNAs were capable of protecting cells against TGEV invasion with very high specificity and efficiency. A real-time quantitative RT-PCR further confirmed that the amounts of viral RNAs in cell cultures pre-transfected with the two plasmids were reduced by 95.2% and up to 100%, respectively. Our results suggest that RNAi might be a promising new strategy against TGEV infection. |
| اللغة: | English |
| تدمد: | 1872-9096 0166-3542 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74e330a5aaaa2b3cff208c1a5ec518a5Test http://europepmc.org/articles/PMC7114347Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....74e330a5aaaa2b3cff208c1a5ec518a5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18729096 01663542 |
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