التفاصيل البيبلوغرافية
| العنوان: |
Glutamate dehydrogenase in plants: is there a new story for an old enzyme? |
| المؤلفون: |
Dubois, Frédéric1, Tercé-Laforgue, Thérèse2, Gonzalez-Moro, Maria-Begoña2,3, Estavillo, José-Maria2,3, Sangwan, Rajbir1, Gallais, André4, Hirel, Bertrand2 hirel@versailles.inra.fr |
| المصدر: |
Plant Physiology & Biochemistry. Jun2003, Vol. 41 Issue 6/7, p565. 12p. |
| مصطلحات موضوعية: |
*DEHYDROGENASES, *PLANTS |
| مستخلص: |
Although good progress has been made to dissect and better understand both the main steps and the regulation of inorganic nitrogen assimilation in higher plants, the role of alternative metabolic pathways which are potentially able to incorporate ammonium into organic molecules is still not fully understood. One of them is the reaction catalysed by the mitochondrial enzyme glutamate dehydrogenase (NAD(H)-GDH, EC 1.4.1.2) which is either able to incorporate ammonium into 2-oxoglutarate to form glutamate or to function in the opposite direction to oxidise glutamate. Although it has been clearly demonstrated by the means of 15N- or 13C-labelling experiments that the later reaction occurs in the cell, it has been argued that under certain physiological conditions, when the ammonium concentration reaches a certain threshold, the enzyme is able to function in the aminating direction. More recently, it has been found that in grapes, a high proportion of the protein is located in the mitochondria of the phloem companion cells and that a significant amount of enzyme is present in the cytosolic fraction of senescing flowers. Using cytoimmunochemistry, we confirmed in the present study that, in other higher plant species, GDH protein is localised in the mitochondria of the phloem companion cells and in the cytosol of senescing organs or tissues. These findings open, therefore, new perspectives toward a better understanding of the function of GDH, particularly in relation to stress and plant development. Both transgenic studies performed in the past and the quantitative genetic approach presented in this paper strongly suggest that the reaction catalysed by NAD(H)-GDH is of major importance in the control of plant growth and productivity. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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