First detection and molecular identification of Borrelia species in Bactrian camel (Camelus bactrianus) from Northwest China
| العنوان: | First detection and molecular identification of Borrelia species in Bactrian camel (Camelus bactrianus) from Northwest China |
|---|---|
| المؤلفون: | Youquan Li, Jianxun Luo, Hong Yin, Zhijie Liu, Yuping Pan, Hongxi Zhao, Qingli Niu, Bintao Zhai, Jifei Yang |
| المصدر: | Infection, Genetics and Evolution |
| بيانات النشر: | Elsevier B.V., 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Veterinary medicine, endocrine system, China, Camelus, relapsing fever, 030106 microbiology, Camelus bactrianus, Microbiology, Polymerase Chain Reaction, Article, Animal Diseases, 03 medical and health sciences, Lyme disease, Borrelia, Genetics, medicine, Animals, Bactrian camel, Geography, Medical, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Phylogeny, Lyme Disease, Borrelia spp, biology, Genes, rRNA, Ribosomal RNA, biology.organism_classification, medicine.disease, 16S ribosomal RNA, bacterial infections and mycoses, 030104 developmental biology, Infectious Diseases, Borrelia Infections, Nested polymerase chain reaction, Nested PCR |
| الوصف: | Comprehensive epidemiological surveys for Lyme disease have not been conducted for the Bactrian camel in China. In this study, a total of 138 blood specimens collected from Bactrian camels from Zhangye City in Gansu Province and Yili and Aksu in Xinjiang Province, China, were examined for the presence of Borrelia spp. Species-specificity nested PCR based on the 5S-23S rRNA, OspA, flaB and 16S rRNA genes revealed that the total positive rate of Borrelia spp. was 3.6% (5/138, 95% CI = 0.2–17.9). These results were confirmed by sequence analysis of the positive PCR products or positive colonies. This is the first report of Borrelia pathogens in camels in China. Two Borrelia species that cause Lyme disease and one that causes relapsing fever were identified in the camel blood samples by sequencing. The findings of this study indicate that the Bactrian camel may serve as a potential natural host of Lyme disease and/or relapsing fever in China. Highlights • This is the first report of Borrelia pathogens using molecular tools in Bactrian camel. • Bactrian camel may play as potential natural host of Lyme disease in China. • The results revealed that the prevalence of Borrelia spp. were 3.6% based on sequences of four genes. • A novel Borrelia genespecies belong to Relapsing fever Borrelia spp. was found. |
| اللغة: | English |
| تدمد: | 1567-7257 1567-1348 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dabe04a614aa93304a0b66320c08fefTest http://europepmc.org/articles/PMC7106200Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3dabe04a614aa93304a0b66320c08fef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15677257 15671348 |
|---|