التفاصيل البيبلوغرافية
| العنوان: |
Anti-proliferative and pro-apoptotic activities of Alpinia oxyphylla on HepG2 cells through ROS-mediated signaling pathway. |
| المؤلفون: |
Zhang, Qiao1,2, Cui, Can1, Chen, Cong-Qin1, Hu, Xiao-Long1, Liu, Ya-Hui1, Fan, Yan-Hua1, Meng, Wei-Hong2, Zhao, Qing-Chun2 zhaoqingchun1967@163.com |
| المصدر: |
Journal of Ethnopharmacology. Jul2015, Vol. 169, p99-108. 10p. |
| مصطلحات موضوعية: |
*PROTEIN metabolism, *ENZYME metabolism, *MEDICINAL plants, *REACTIVE oxygen species, *ALTERNATIVE medicine, *ANTINEOPLASTIC agents, *APOPTOSIS, *BIOLOGICAL assay, *BIOLOGICAL transport, *BREAST tumors, *CELL migration, *CELLULAR signal transduction, *COLON tumors, *ENDOTHELIUM, *FLOW cytometry, *HIGH performance liquid chromatography, *LACTATE dehydrogenase, *LEUKEMIA, *LIVER tumors, *MICROBIOLOGICAL assay, *MICROSCOPY, *MITOCHONDRIA, *STAINS & staining (Microscopy), *UMBILICAL cord, *WESTERN immunoblotting, *PLANT extracts, *DESCRIPTIVE statistics, *IN vitro studies, *PHARMACODYNAMICS |
| مصطلحات جغرافية: |
ASIA |
| مستخلص: |
Ethnopharmacological relevance Fructus Alpiniae oxyphyllae ( A. oxyphylla ) is a traditional herb which is widely used in East Asian for the treatment of dyspepsia, diarrhea, abdominal pain, poor memory, inflammatory conditions and cancer. Materials and methods The cytotoxic activities of ethanol extract (EE) and five extract layers including petroleum ether (PE), dichloromethane (DCLM), acetoacetate (EtOAc), n -Butanol (n-Bu) and water fractions (WF) of A. oxyphylla were tested on HepG2, SW480, MCF-7, K562 and HUVEC cell lines using MTT assay and LDH release assay. The component analysis was performed on HPLC with gradient elution. Hoechst 33342 staining, DCFH-DA fluorescence microscopy, flow cytometry analysis, western blot and migration assays were carried out to determine the anti-cancer mechanisms of PE. Results MTT analysis showed that EE, PE and DCLM could inhibit cell proliferation on HepG2, SW480, MCF-7, K562 and HUVEC cell lines, especially PE fraction. HPLC analysis pointed out five main components which may contribute to the anti-proliferative activity of PE. Further study showed that PE increased LDH release, induced apoptosis, disrupted mitochondrial membrane potential and elevated intracellular reactive oxygen species (ROS) in HepG2 cells, whereas the antioxidant N-acetyl-l-cysteine (NAC) prevented PE-induced ROS generation. The results of western blot revealed that PE induced apoptosis in HepG2 cells by enhancing Bax/Bcl-2 ratio, increasing cytochrome c in cytosol and activating caspase-3/9. Meanwhile, high levels of ROS could induce DNA damage-mediated protein expression, AKT, ERK inactivation and SAPKs activation. Furthermore, PE conspicuously blocked the migration of HUVEC cells. Conclusion The present results demonstrated that PE induced apoptosis in HepG2 cells may be via a ROS-mediated signaling pathway. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
