التفاصيل البيبلوغرافية
| العنوان: |
Expression of the phospholipid scramblase (PLSCR) gene family during the acute phase response |
| المؤلفون: |
Lu, Biao1 lubiao@gmail.com, Sims, Peter J.2, Wiedmer, Therese2, Moser, Arthur H.1, Shigenaga, Judy K.1, Grunfeld, Carl1, Feingold, Kenneth R.1 |
| المصدر: |
BBA - Molecular & Cell Biology of Lipids. Sep2007, Vol. 1771 Issue 9, p1177-1185. 9p. |
| مصطلحات موضوعية: |
*CYTOKINES, *CYTOLOGY, *PHOSPHOLIPIDS, *LIPIDS |
| مستخلص: |
Abstract: Phospholipid scramblase 1 (PLSCR1) is a member of PLSCR gene family that has been implicated in multiple cellular processes including movement of phospholipids, gene regulation, immuno-activation, and cell proliferation/apoptosis. In the present study, we identified PLSCR1 as a positive intracellular acute phase protein that is upregulated by LPS in liver, heart, and adipose tissue, but not skeletal muscle. LPS administration resulted in a marked increase in PLSCR1 mRNA and protein levels in the liver. This stimulation occurred rapidly (within 2 h), and was very sensitive to LPS (half-maximal response at 0.1 μg/mouse). Moreover, two other APR-inducers, zymosan and turpentine, also produced significant increases in PLSCR1 mRNA and protein levels, indicating that PLSCR1 was stimulated in a number of models of the APR. To determine signaling pathways by which LPS stimulated PLSCR1, we examined the effect of proinflammatory cytokines in vitro and in vivo. TNFα, IL-1β, and IL-6 all stimulated PLSCR1 in cultured Hep B3 hepatocytes, whereas only TNFα stimulated PLSCR1 in cultured 3T3-L1 adipocytes, suggesting cell type-specific effects of cytokines. Furthermore, the LPS-stimulated increase in liver PLSCR1 mRNA was greatly attenuated by 80% in TNFα and IL-1β receptor null mice as compared to wild-type controls. In contrast, PLSCR1 levels in adipose tissue were induced to a similar extent in TNFα and IL-1β receptor null mice and controls. These results indicate that maximal stimulation of PLSCR1 by LPS in liver required TNFα and/or IL-1β, whereas the stimulation of PLSCR1 in adipose tissue is not dependent on TNFα and/or IL-1β. These data provide evidence that PLSCR1 is a positive intracellular acute phase protein with a tissue-specific mechanism for up-regulation. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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