التفاصيل البيبلوغرافية
| العنوان: |
Engineering a biomimetic three-dimensional nanostructured bone model for breast cancer bone metastasis study. |
| المؤلفون: |
Zhu, Wei, Wang, Mian, Fu, Yebo, Castro, Nathan J., Fu, Sidney W., Zhang, Lijie Grace |
| المصدر: |
Acta Biomaterialia; Mar2015, Vol. 14, p164-174, 11p |
| مصطلحات موضوعية: |
BREAST cancer, BIOMIMETIC materials, BONE metastasis, BIOCOMPATIBILITY, HYDROXYAPATITE, CHITOSAN |
| مستخلص: |
Traditional breast cancer (BrCa) bone metastasis models contain many limitations with regards to controllability, reproducibility and flexibility of design. In this study, a novel biomimetic bone microenvironment was created by integrating hydroxyapatite (HA) and native bioactive factors deposited by osteogenic induction of human bone marrow mesenchymal stem cells (MSCs) within a cytocompatible chitosan hydrogel. It was found that a 10% nanocrystalline HA (nHA) chitosan scaffold exhibited the highest BrCa adhesion and proliferation when compared to chitosan scaffolds with 20% nHA, 10% and 20% microcrystalline HA as well as amorphous HA. This 3-D tunable bone scaffold can provide a biologically relevant environment, increase cell–cell and cell–matrix interactions as found in native bone, and retain the behavior of BrCa cells with different metastasis potential (i.e. highly metastatic MDA-MB-231, less metastatic MCF-7 and transfected MDA-MB-231). The co-culture of MSCs and MDA-MB-231 in this bone model illustrated that MSCs have the capacity to upregulate the expression of the well-known metastasis-associated gene metadherin within BrCa cells. In summary, this study illustrates the ability of our 3-D bone model to create a biomimetic environment conducive to recapitulating the behavior of metastatic BrCa cells, making it a promising tool for in vitro BrCa cell bone metastasis study and for the discovery of potential therapeutics. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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