التفاصيل البيبلوغرافية
| العنوان: |
Protective effects of peony glycosides against corticosterone-induced cell death in PC12 cells through antioxidant action |
| المؤلفون: |
Mao, Qing-Qiu1, Xian, Yan-Fang1, Ip, Siu-Po paulip@cuhk.edu.hk, Tsai, Sam-Hip1, Che, Chun-Tao1 |
| المصدر: |
Journal of Ethnopharmacology. Feb2011, Vol. 133 Issue 3, p1121-1125. 5p. |
| مصطلحات موضوعية: |
*ENZYME analysis, *REACTIVE oxygen species, *ALTERNATIVE medicine, *ANALYSIS of variance, *ANIMAL experimentation, *ANTIOXIDANTS, *BIOLOGICAL assay, *BIOLOGICAL models, *BIOPHYSICS, *BRAIN, *DOSE-effect relationship in pharmacology, *FLOWERS, *GLUTATHIONE, *GLYCOSIDES, *LACTATE dehydrogenase, *RESEARCH methodology, *MEDICINAL plants, *RATS, *RESEARCH funding, *STATISTICS, *SUPEROXIDE dismutase, *MALONDIALDEHYDE, *PLANT extracts, *DATA analysis |
| مصطلحات جغرافية: |
HONG Kong (China) |
| مستخلص: |
Abstract: Aim of the study: Previous studies in our laboratory have shown that total glycosides of peony (TGP) produced antidepressant-like action in various mouse models of behavioral despair. However, the molecular mechanism by which TGP exerts antidepressant-like effect is not fully understood. This study examined the protective ffects of TGP against corticosterone-induced neurotoxicity in rat pheochromocytoma (PC12) cells and ts possible mechanisms. Materials and methods: The direct antioxidant effect of TGP was investigated by using a 2,2′-azinobis-(3-ethylbenzothiazoline- 6-sulphonic acid) (ABTS) radical cation-scavenging assay in a cell-free system. PC12 cells were treated with 200 μM of corticosterone in the absence or presence of TGP in varying concentrations for 48h. Cell viability, lactate dehydrogenase (LDH) activity, intracellular reactive oxygen species (ROS) level, malondialdehyde (MDA) content, glutathione (GSH) content, superoxide dismutase (SOD) activity, and catalase (CAT) activity were then determined. Results: TGP displayed antioxidant properties in the cell-free system, and the IC50 value in the ABTS radical cation-scavenging assay was 9.9mg/L. TGP treatment at increasing doses (1–10mg/L) protected against corticosterone-induced cytotoxicity in PC12 cells in a dose-dependent manner. The cytoprotection afforded by TGP treatment was associated with decreases in the intracellular ROS and MDA levels, and increases in the GSH level, SOD activity, and CAT activity in corticosterone-treated PC12 cells. Conclusion: The results suggest that TGP has a neuroprotective effect on corticosterone-induced neurotoxicity in PC12 cells, which may be related to its antioxidant action. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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