Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation
| العنوان: | Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
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| المؤلفون: | Francisco Peixoto, Filipe V. Duarte, Ana P. Gomes, Ana T. Varela, Anabela P. Rolo, Carlos M. Palmeira, João S. Teodoro |
| المصدر: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP CIÊNCIAVITAE |
| بيانات النشر: | Elsevier B.V., 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | medicine.medical_specialty, SIRT3, Berberine, SirT3, Mitochondria, Liver, Oxidative phosphorylation, Mitochondrion, Diet, High-Fat, Real-Time Polymerase Chain Reaction, Oxidative Phosphorylation, 03 medical and health sciences, chemistry.chemical_compound, Adenosine Triphosphate, 0302 clinical medicine, AMP-activated protein kinase, Sirtuin 3, Internal medicine, medicine, Animals, Obesity, Molecular Biology, DNA Primers, 030304 developmental biology, Membrane Potential, Mitochondrial, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, Base Sequence, biology, Acetyl-CoA carboxylase, Cell Biology, Rats, Endocrinology, chemistry, 030220 oncology & carcinogenesis, Sirtuin, Liver bioenergetics, biology.protein, Molecular Medicine, Mitochondrial function, Reactive Oxygen Species |
| الوصف: | Berberine is an isoquinoline alkaloid with anti-diabetic properties. Despite the central role of liver and thus hepaticmitochondria inwhole-bodymetabolism, berberine effects on hepaticmitochondrial function in an obesity model are still unknown. Here, we demonstrate that berberine treatment recovers mitochondrial efficiency when altered by a high-fat feeding.Mitochondria isolated from the liver of high-fat fed rats exhibited decreased capacity to accumulate calcium and impaired oxidative phosphorylation (OXPHOS) capacity, as shown by impaired mitochondrialmembrane potential, oxygen consumption and cellular ATP levels. Interestingly, the recovery of mitochondrial function by berberine was associated with an increased activity of the mitochondrial sirtuin 3 (SirT3). In conclusion, berberine potent protective effects against metabolic syndrome may rely on increasing mitochondrial SirT3 activity, normalizing mitochondrial function and preventing a state of energetic deficit caused by impaired OXPHOS. JST, FVD, APG and ATV were recipients of a Fundação para a Ciência e a Tecnologia PhD scholarship (SFRH/BD/38467/2007, SFRH/BD/38372/ 2007, SFRH/BD/44674/2008 and SFRH/BD/44796/2008, respectively). This project was supported by a FCT grant PTCD/SAU-OSM/72443/ 2006. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80191fc07767876de0365da9478dc164Test https://hdl.handle.net/10316/25615Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....80191fc07767876de0365da9478dc164 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |