دورية أكاديمية
Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells
| العنوان: | Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells |
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| المؤلفون: | Zvick, Joel, Tarnowska-Sengül, Monika, Ghosh, Adhideb, id_orcid:0 000-0002-5160-4571, Bundschuh, Nicola, Gjonlleshaj, Pjeter, Hinte, Laura, id_orcid:0 000-0002-4226-0009, Trautmann, Christine L., Noé, Falko, Qabrati, Xhem, Domenig, Seraina A., Kim, Inseon, Hennek, Thomas, von Meyenn, Ferdinand, id_orcid:0 000-0001-9920-3075, Bar-Nur, Ori, id_orcid:0 000-0002-6466-3124 |
| المصدر: | Stem Cell Reports, 17 (9) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | ETH Zürich Research Collection |
| مصطلحات موضوعية: | Stem cell biology, Germ cell production, pluripotency, chimerism |
| الوصف: | Blastocyst complementation denotes a technique that aims to generate organs, tissues, or cell types in animal chimeras via injection of pluripotent stem cells (PSCs) into genetically compromised blastocyst-stage embryos. Here, we report on successful complementation of the male germline in adult chimeras following injection of mouse or rat PSCs into mouse blastocysts carrying a mutation in Tsc22d3, an essential gene for spermatozoa production. Injection of mouse PSCs into Tsc22d3-Knockout (KO) blastocysts gave rise to intraspecies chimeras exclusively embodying PSC-derived functional spermatozoa. In addition, injection of rat embryonic stem cells (rESCs) into Tsc22d3-KO embryos produced interspecies mouse-rat chimeras solely harboring rat spermatids and spermatozoa capable of fertilizing oocytes. Furthermore, using single-cell RNA sequencing, we deconstructed rat spermatogenesis occurring in a mouse-rat chimera testis. Collectively, this study details a method for exclusive xenogeneic germ cell production in vivo, with implications that may extend to rat transgenesis, or endangered animal species conservation efforts. ; ISSN:2213-6711 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/000860986300004; info:eu-repo/grantAgreement/SNF/Eccellenza/187009; info:eu-repo/grantAgreement/EC/H2020/803491; http://hdl.handle.net/20.500.11850/564133Test |
| DOI: | 10.3929/ethz-b-000564133 |
| الإتاحة: | https://doi.org/20.500.11850/564133Test https://doi.org/10.3929/ethz-b-000564133Test https://doi.org/10.1016/j.stemcr.2022.07.005Test https://hdl.handle.net/20.500.11850/564133Test |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
| رقم الانضمام: | edsbas.78224C7A |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000564133 |
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