دورية أكاديمية
Cytotoxicity of CeO2 nanoparticles using in vitro assay with Mytilus galloprovincialis hemocytes: Relevance of zeta potential, shape and biocorona formation
| العنوان: | Cytotoxicity of CeO2 nanoparticles using in vitro assay with Mytilus galloprovincialis hemocytes: Relevance of zeta potential, shape and biocorona formation |
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| المؤلفون: | Sendra, M, Volland, M, BALBI, TERESA, FABBRI, RITA, Yeste, M P, Gatica, J M, CANESI, LAURA, Blasco, J |
| المساهمون: | Sendra, M, Volland, M, Balbi, Teresa, Fabbri, Rita, Yeste, M P, Gatica, J M, Canesi, Laura, Blasco, J |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | Università degli Studi di Genova: CINECA IRIS |
| مصطلحات موضوعية: | Agglomeration, Biocorona, CeO(2) NPs, Immune regulation, Mussel, Zeta potential |
| الوصف: | Over the last decades, the growth in nanotechnology has provoked an increase in the number of its applications and consumer products that incorporate nanomaterials in their formulation. Metal nanoparticles are released to the marine environment and they can interact with cells by colloids forces establish a nano-bio interface. This interface can be compatible or generate bioadverse effects to cells. The daily use of CeO2 nanoparticles (CeO2 NPs) in industrial catalysis, sunscreen, fuel cells, fuel additives and biomedicine and their potential release into aquatic environments has turned them into a new emerging pollutant of concern. It is necessary to assess of effects of CeO2 NPs in aquatic organisms and understand the potential mechanisms of action of CeO2 NP toxicity to improve our knowledge about the intrinsic and extrinsic characteristic of CeO2 NPs and the interaction of CeO2 NPs with biomolecules in different environment and biological fluids. The conserved innate immune system of bivalves represents a useful tool for studying immunoregulatory responses when cells are exposed to NPs. In this context, the effects of two different CeO2 NPs with different physico-chemical characteristics (size, shape, zeta potential and Ce+3/Ce+4 ratio) and different behavior with biomolecules in plasma fluid were studied in a series of in vitro assays using primary hemocytes from Mytilus galloprovincialis. Different cellular responses such as lysosome membrane stability, phagocytosis capacity and extracellular reactive oxygen species (ROS) production were evaluated. Our results indicate that the agglomeration state of CeO2 NPs in the exposure media did not appear to have a substantial role in particle effects, while differences in shape, zeta potential and biocorona formation in NPs appear to be important in provoking negative impacts on hemocytes. The negative charge and the rounded shape of CeO2 NPs, which formed Cu, Zn-SOD biocorona in hemolymph serum (HS), triggered higher changes in the biomarker of stress (LMS) and ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/29704629; info:eu-repo/semantics/altIdentifier/wos/WOS:000438180700002; volume:200; firstpage:13; lastpage:20; numberofpages:8; journal:AQUATIC TOXICOLOGY; https://hdl.handle.net/11567/907923Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85046147072 |
| DOI: | 10.1016/j.aquatox.2018.04.011 |
| الإتاحة: | https://doi.org/10.1016/j.aquatox.2018.04.011Test https://hdl.handle.net/11567/907923Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.66CD1263 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.aquatox.2018.04.011 |
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