دورية أكاديمية
Validation of whole-slide digitally imaged melanocytic lesions: Does z-stack scanning improve diagnostic accuracy?
| العنوان: | Validation of whole-slide digitally imaged melanocytic lesions: Does z-stack scanning improve diagnostic accuracy? |
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| المؤلفون: | Bart Sturm, David Creytens, Martin G Cook, Jan Smits, Marcory C. R. F. van Dijk, Erik Eijken, Eline Kurpershoek, Heidi V. N Küsters-Vandevelde, Ariadne H. A. G. Ooms, Carla Wauters, Willeke A. M. Blokx, Jeroen A. W. M. van der Laak |
| المصدر: | Journal of Pathology Informatics, Vol 10, Iss 1, Pp 6-6 (2019) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Melanoma, validation, whole-slide image, whole-slide imaging, z-stack, Computer applications to medicine. Medical informatics, R858-859.7, Pathology, RB1-214 |
| الوصف: | Background: Accurate diagnosis of melanocytic lesions is challenging, even for expert pathologists. Nowadays, whole-slide imaging (WSI) is used for routine clinical pathology diagnosis in several laboratories. One of the limitations of WSI, as it is most often used, is the lack of a multiplanar focusing option. In this study, we aim to establish the diagnostic accuracy of WSI for melanocytic lesions and investigate the potential accuracy increase of z-stack scanning. Z-stack enables pathologists to use a software focus adjustment, comparable to the fine-focus knob of a conventional light microscope. Materials and Methods: Melanocytic lesions (n = 102) were selected from our pathology archives: 35 nevi, 5 spitzoid tumors of unknown malignant potential, and 62 malignant melanomas, including 10 nevoid melanomas. All slides were scanned at a magnification comparable to use of a ×40 objective, in z-stack mode. A ground truth diagnosis was established on the glass slides by four academic dermatopathologists with a special interest in the diagnosis of melanoma. Six nonacademic surgical pathologists subspecialized in dermatopathology examined the cases by WSI. Results: An expert consensus diagnosis was achieved in 99 (97%) of cases. Concordance rates between surgical pathologists and the ground truth varied between 75% and 90%, excluding nevoid melanoma cases. Concordance rates of nevoid melanoma varied between 10% and 80%. Pathologists used the software focusing option in 7%–28% of cases, which in 1 case of nevoid melanoma resulted in correcting a misdiagnosis after finding a dermal mitosis. Conclusion: Diagnostic accuracy of melanocytic lesions based on glass slides and WSI is comparable with previous publications. A large variability in diagnostic accuracy of nevoid melanoma does exist. Our results show that z-stack scanning, in general, does not increase the diagnostic accuracy of melanocytic. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2153-3539 |
| العلاقة: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2019;volume=10;issue=1;spage=6;epage=6;aulast=SturmTest; https://doaj.org/toc/2153-3539Test; https://doaj.org/article/a97fb7ed54fd4943b24677232d1e903aTest |
| DOI: | 10.4103/jpi.jpi_46_18 |
| الإتاحة: | https://doi.org/10.4103/jpi.jpi_46_18Test https://doaj.org/article/a97fb7ed54fd4943b24677232d1e903aTest |
| رقم الانضمام: | edsbas.48CE26E4 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21533539 |
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| DOI: | 10.4103/jpi.jpi_46_18 |