دورية أكاديمية
Molecular signatures of medullary thyroid carcinoma by matrix-assisted laser desorption/ionisation mass spectrometry imaging
| العنوان: | Molecular signatures of medullary thyroid carcinoma by matrix-assisted laser desorption/ionisation mass spectrometry imaging |
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| المؤلفون: | Smith, Andrew, Galli, Manuel, Piga, Isabella, Denti, Vanna, Stella, Martina, Chinello, Clizia, Fusco, Nicola, Leni, Davide, Manzoni, Marco, Roversi, Gaia, Garancini, Mattia, Pincelli, Angela Ida, Cimino, Vincenzo, Capitoli, Giulia, Magni, Fulvio, Pagni, Fabio |
| المساهمون: | A. Smith, M. Galli, I. Piga, V. Denti, M. Stella, C. Chinello, N. Fusco, D. Leni, M. Manzoni, G. Roversi, M. Garancini, A.I. Pincelli, V. Cimino, G. Capitoli, F. Magni, F. Pagni |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | C cell hyperplasia, Cancer, Mass spectrometry imaging, Medullary thyroid carcinoma, proteomic, biophysic, biochemistry, Settore MED/08 - Anatomia Patologica |
| الوصف: | The main aim of the study was to assess the feasibility of matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) in the pathological investigation of Medullary Thyroid Carcinoma (MTC). Formalin-fixed paraffin-embedded (FFPE) samples from seven MTC patients were analysed by MALDI-MSI in order to detect proteomic alterations within tumour lesions and to define the molecular profiles of specific findings, such as amyloid deposition and C cell hyperplasia (CCH). nLC-ESI MS/MS was employed for the identification of amyloid components and to select alternative proteomic markers of MTC pathogenesis. Results highlighted the potential of MALDI-MSI to confirm the classic immunohistochemical methods employed for the diagnosis of MTC, with good sensitivity and specificity. Intratumoural amyloid components were also detected and identified, and were characterised by calcitonin, apolipoprotein E, apolipoprotein IV, and vitronectin. The tryptic peptide profiles representative of MTC and CCH were distinctly different, with four alternative markers for MTC being detected; K1C18, and three histones (H2A, H3C, and H4). Finally, a further 115 proteins were identified through the nLC-ESI-MS/MS analysis alone, with moesin, veriscan, and lumican being selected due to their potential involvement in MTC pathogenesis. This approach represents a complimentary strategy that could be employed to detect new proteomic markers of MTC. Statement of significance: Medullary thyroid carcinoma (MTC) is a rare endocrine malignancy that originates from the parafollicular C-cells of the thyroid. The diagnosis is typically established using a combination of fine-needle aspiration biopsy (FNAB) of a suspicious nodule along with the demonstrable elevation of serum biomarkers, such as calcitonin and carcinoembryonic antigen (CEA). Unfortunately, this combination is often associated with a high degree of false-positive results and this can lead to misdiagnosis and avoidable total thyroidectomy. The current study presents the ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/29581064; info:eu-repo/semantics/altIdentifier/wos/WOS:000454963700013; numberofpages:10; journal:JOURNAL OF PROTEOMICS; http://hdl.handle.net/2434/578230Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85044586054 |
| DOI: | 10.1016/j.jprot.2018.03.021 |
| الإتاحة: | https://doi.org/10.1016/j.jprot.2018.03.021Test http://hdl.handle.net/2434/578230Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.1A42479D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jprot.2018.03.021 |
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