المؤلفون: Hoai-Nam Truong, Carine Fournier, Siham Hichami, Léna Dole, Fanny Leroy, David Wendehenne, Valérie Nicolas-Francès, Angélique Besson-Bard

المصدر: Plant Stress, Vol 12, Iss , Pp 100488- (2024)

مصطلحات موضوعية: Iron, Nutrient stress, Reactive oxygen species, Remobilization, Siderophore, Plant ecology, QK900-989

الوصف: Iron (Fe) is an essential micronutrient for most organisms that is, however, poorly bioavailable in the soil. Acquiring this element is therefore a major issue for both plants and microorganisms. Bacteria acquire Fe by secreting siderophores that chelate the ferric ion with a high affinity and that can contribute to Fe nutrition in plants, under certain conditions including stressful ones. Our previous work has shown that pyoverdine, a siderophore purified from a beneficial Pseudomonas sp., leads to plant growth stimulation when supplied in its Fe-free form (apo-pyoverdine/apopyo) to Arabidopsis thaliana plants grown hydroponically under Fe-deficient conditions. To better understand siderophore-mediated growth promotion, we studied the impact of an apo-siderophore with a different structure from pyoverdine, deferoxamine (DFO), on the same model. Here we show that growth stimulation is linked to the ability of siderophores to chelate Fe, as DFO also promotes growth after 3 to 6 days when provided at 25 µM to 4-week-old A. thaliana plants starved for Fe for 1 to 7 days. The extent to which the siderophore stimulates root and shoot growth depends on the amount of Fe stored in the roots prior to the addition of DFO, and likely involves the remobilization of Fe from the roots to the shoots. The promotion of root growth by DFO does not appear to depend on coumarin production or secretion, but is associated with an increase in the expression of genes of the HOMOLOG OF BEE2 INTERACTING WITH IBH1 (HBI1)-dependent brassinosteroid signaling pathway. DFO stimulates lateral root elongation concomitant with a significant decrease in hydrogen peroxide (H2O2) and oxidized glutathione levels and a decrease in stress-related gene expression in roots. In addition, DFO decreases the expression of the UPBEAT (UPB1) gene involved in H2O2-mediated root growth inhibition, and thus lifts the inhibition of the expression of its target genes, the class III peroxidases that stimulate root growth. Overall, this work indicates that growth promotion by apo-siderophores in an iron-poor environment is likely linked to their ability to induce a stronger response to Fe deficiency while decreasing oxidative stress in roots, thus stimulating Fe remobilization from root Fe pools to shoots and promoting secondary root growth.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2667064X24001428Test; https://doaj.org/toc/2667-064XTest

الوصول الحر: https://doaj.org/article/5dbe196afab74e64a1cc4fa6b205d580Test

المؤلفون: Vatsa, Parul, Chiltz, Annick, Bourque, Stephane, Wendehenne, David, Garcia-Brugger, Angela, Pugin, Alain

المساهمون: Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), The authors would like to thank Christine Arnould for confocal microscopy experiments, and Siham Hichami for technical assistance. This work was partly funded by l'Agence Nationale de la Recherche (ANR) CaPhe and supported by the GDR CNRS 2688 "Calcium and gene expression in normal and pathological situations". The authors also acknowledge funding support from the Conseil Regional de Bourgogne.

المصدر: ISSN: 0300-9084 ; Biochimie ; https://hal.inrae.fr/hal-02652327Test ; Biochimie, 2011, 93 (12), pp.2095-2101. ⟨10.1016/j.biochi.2011.04.006⟩.

مصطلحات موضوعية: calcium signaling, plant defence, tobacco, elicitors, biochemistry and molecular biology, glutamate receptor, nitric oxide, résistance végétale, tabac, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology

الوصف: International audience ; Ionotropic glutamate receptors (iGluRs) are non-selective cation channels permeable to calcium, present in animals and plants. In mammals, glutamate is a well-known neurotransmitter and recently has been recognized as an immunomodulator. As animals and plants share common mechanisms that govern innate immunity with calcium playing a key role in plant defence activation, we have checked the involvement of putative iGluRs in plant defence signaling. Using tobacco cells, we first provide evidence supporting the activity of iGluRs as calcium channels and their involvement in NO production as reported in animals. Thereafter, iGluRs were shown to be activated in response to cryptogein, a well studied elicitor of defence response, and partly responsible for cryptogein-induced NO production. However, other cryptogein-induced calcium-dependent events including anion efflux, H2O2 production, MAPK activation and hypersensitive response (HR) did not depend on iGluRs indicating that different calcium channels regulate different processes at the cell level. We have also demonstrated that cryptogein induces efflux of glutamate in the apoplast by exocytosis. Taken together, our results demonstrate for the first time, an involvement of a putative iGluR in plant defence signaling and NO production, by mechanisms that show homology with glutamate mode of action in mammals.

العلاقة: hal-02652327; https://hal.inrae.fr/hal-02652327Test; PRODINRA: 45549; WOS: 000297887300011

الإتاحة: https://doi.org/10.1016/j.biochi.2011.04.006Test
https://hal.inrae.fr/hal-02652327Test