دورية أكاديمية
Abnormal Peyer patch development and B-cell gut homing drive IgA deficiency in Kabuki syndrome.
| العنوان: | Abnormal Peyer patch development and B-cell gut homing drive IgA deficiency in Kabuki syndrome. |
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| المؤلفون: | Pilarowski, Genay O, Cazares, Tareian, Zhang, Li, Benjamin, Joel S, Liu, Ke, Jagannathan, Sajjeev, Mousa, Nadeem, Kasten, Jennifer, Barski, Artem, Lindsley, Andrew W, Bjornsson, Hans T |
| المساهمون: | 1Predoctoral Program in Human Genetics, Johns Hopkins School of Medicine, Baltimore, Md, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Md. 2Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio. 3McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Md. 4Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio. 5Division of Pathology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio. 6Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio. 7Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio. Electronic address: andrew.lindsley@cchmc.org. 8McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Md, Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore, Md, Faculty of Medicine, University of Iceland, Reykjavik, Iceland, Department of Genetics and Molecular Biology, Landspitali University Hospital, Reykjavik, Iceland. |
| المصدر: | The Journal of allergy and clinical immunology ; 145 ; 3 ; 982 ; 992 ; United States |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | Hirsla - Landspítali University Hospital research archive |
| مصطلحات موضوعية: | B-cell maturation, B1 B cells, ITGB7, IgA, KMT2D, epigenetics, gut lymphocyte homing, hypogammaglobulinemia, Kabuki syndrome, Kabuki heilkenni |
| الوصف: | To access publisher's full text version of this article click on the hyperlink below ; Background: Kabuki syndrome (KS) is commonly caused by mutations in the histone-modifying enzyme lysine methyltransferase 2D (KMT2D). Immune dysfunction is frequently observed in individuals with KS, but the role of KMT2D in immune system function has not been identified. Objective: We sought to understand the mechanisms driving KS-associated immune deficiency (hypogammaglobulinemia [low IgA], splenomegaly, and diminished immunization responses). Methods: We performed a comprehensive evaluation of humoral immunity and secondary lymphoid tissues in an established KS (Kmt2d+/βGeo) mouse model and validated select findings in a patient with KS. Results: Compared with wild-type littermates, Kmt2d+/βGeo mice demonstrated deficiencies in multiple B-cell lineages and reduced serum IgA and elevated IgM levels across multiple ages. The bone marrow, spleen, and intestine of Kmt2d+/βGeo mice contained diminished numbers of IgA-secreting cells, while elevated germinal center B cells were found in the mesenteric lymph node and Peyer patches. Kmt2d+/βGeo mice have decreased size and numbers of Peyer patches, a finding confirmed in human samples. We identified deficiency of Itgb7 RNA and protein expression, a gene encoding an adhesion protein that mediates intestinal homing, and we demonstrated KMT2D-dependent control of ITGB7 expression in a human cell line. Conclusions: Kmt2d haploinsufficiency has broad deleterious effects on B-cell differentiation, specifically hampering gut lymphocyte homing and IgA+ plasma cell differentiation. Intestinal lymphoid defects caused by ITGB7 deficiency have not previously been recognized in KS, and these results provide new mechanistic insights into the pathogenesis of KS-associated immune deficiency. ; Center for Pediatric Genomics, Cincinnati Children's Research Foundation, United States Department of Health & Human Services National Institutes of Health (NIH) - USA, Louma G. Foundation |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1097-6825 |
| العلاقة: | https://www.sciencedirect.com/science/article/pii/S0091674919316276?via%3DihubTest; Pilarowski GO, Cazares T, Zhang L, et al. Abnormal Peyer patch development and B-cell gut homing drive IgA deficiency in Kabuki syndrome. J Allergy Clin Immunol. 2020;145(3):982‐992. doi:10.1016/j.jaci.2019.11.034; http://hdl.handle.net/2336/621381Test; The Journal of allergy and clinical immunology |
| DOI: | 10.1016/j.jaci.2019.11.034 |
| الإتاحة: | https://doi.org/10.1016/j.jaci.2019.11.034Test http://hdl.handle.net/2336/621381Test |
| حقوق: | Copyright © 2019 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved. ; National Consortium - Landsaðgangur |
| رقم الانضمام: | edsbas.24CBDAFC |
| قاعدة البيانات: | BASE |
| تدمد: | 10976825 |
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| DOI: | 10.1016/j.jaci.2019.11.034 |