دورية أكاديمية
Morphology and structure of lipoproteins revealed by an optimized negative-staining protocol of electron microscopy
| العنوان: | Morphology and structure of lipoproteins revealed by an optimized negative-staining protocol of electron microscopy |
|---|---|
| المؤلفون: | Zhang, L, Song, J, Cavigiolio, G, Ishida, BY, Zhang, S, Kane, JP, Weisgraber, KH, Oda, MN, Rye, K-A, Pownall, HJ, Ren, G |
| بيانات النشر: | ELSEVIER |
| سنة النشر: | 2011 |
| المجموعة: | The University of Melbourne: Digital Repository |
| الوصف: | Plasma lipoprotein levels are predictors of risk for coronary artery disease. Lipoprotein structure-function relationships provide important clues that help identify the role of lipoproteins in cardiovascular disease. The compositional and conformational heterogeneity of lipoproteins are major barriers to the identification of their structures, as discovered using traditional approaches. Although electron microscopy (EM) is an alternative approach, conventional negative staining (NS) produces rouleau artifacts. In a previous study of apolipoprotein (apo)E4-containing reconstituted HDL (rHDL) particles, we optimized the NS method in a way that eliminated rouleaux. Here we report that phosphotungstic acid at high buffer salt concentrations plays a key role in rouleau formation. We also validate our protocol for analyzing the major plasma lipoprotein classes HDL, LDL, IDL, and VLDL, as well as homogeneously prepared apoA-I-containing rHDL. High-contrast EM images revealed morphology and detailed structures of lipoproteins, especially apoA-I-containing rHDL, that are amenable to three-dimensional reconstruction by single-particle analysis and electron tomography. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0022-2275 1539-7262 |
| العلاقة: | pii: S0022-2275(20)40509-7; Zhang, L., Song, J., Cavigiolio, G., Ishida, B. Y., Zhang, S., Kane, J. P., Weisgraber, K. H., Oda, M. N., Rye, K. -A., Pownall, H. J. & Ren, G. (2011). Morphology and structure of lipoproteins revealed by an optimized negative-staining protocol of electron microscopy. JOURNAL OF LIPID RESEARCH, 52 (1), pp.175-184. https://doi.org/10.1194/jlr.D010959Test.; http://hdl.handle.net/11343/264011Test |
| الإتاحة: | https://doi.org/10.1194/jlr.D010959Test http://hdl.handle.net/11343/264011Test |
| حقوق: | CC BY-NC ; https://creativecommons.org/licenses/by-nc/4.0Test |
| رقم الانضمام: | edsbas.5E6469 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00222275 15397262 |
|---|