دورية أكاديمية
Neuronal and glial glycine transporters have different stoichiometries.
| العنوان: | Neuronal and glial glycine transporters have different stoichiometries. |
|---|---|
| المؤلفون: | Roux, Michel, J., Supplisson, Stéphane |
| المساهمون: | Laboratoire de Neurobiologie (UMR 8544) (NEURO), École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), We are grateful to Philippe Ascher for his encouragement, support and invaluable comments on the manuscript. Thanks to Boris Barbour for critically reading and comments on the manuscript, to Stéphane Dieudonné, Baruch Kanner and Donald F. Loo for stimulating discussions, and to Anne Le Goff for technical assistance. This work was supported by the CNRS and a grant from the European Community (contract BMH4CT950571). |
| المصدر: | ISSN: 0896-6273 ; Neuron ; https://hal.science/hal-00140070Test ; Neuron, 2000, 25 (2), pp.373-83. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2000 |
| المجموعة: | Inserm: HAL (Institut national de la santé et de la recherche médicale) |
| مصطلحات موضوعية: | GlyT1, GlyT2, NMDA, stoichiometries, Na+, transporter, glycine, thermodynamics, flux coupling, MESH: Amino Acid Transport Systems, Neutral, MESH: Animals, MESH: Glycine, MESH: Glycine Plasma Membrane Transport Proteins, MESH: Kinetics, MESH: Membrane Potentials, MESH: Neuroglia, MESH: Neurons, MESH: Biological Transport, MESH: Carrier Proteins, MESH: Electric Conductivity, MESH: Electrophysiology, MESH: Extracellular Space, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology |
| الوصف: | A neurotransmitter transporter can potentially mediate uptake or release of substrate, and its stoichiometry is a key factor that controls the driving force and thus the neurotransmitter flux direction. We have used a combination of electrophysiology and radio-tracing techniques to evaluate the stoichiometries of two glycine transporters involved in glycinergic or glutamatergic transmission. We show that GlyT2a, a transporter present in glycinergic boutons, has a stoichiometry of 3 Na+/Cl-/glycine, which predicts effective glycine accumulation in all physiological conditions. GlyT1b, a glial transporter, has a stoichiometry of 2 Na+/Cl-/ glycine, which predicts that glycine can be exported or imported, depending on physiological conditions. GlyT1b may thus modulate glutamatergic synapses by increasing or decreasing the glycine concentration around N-methyl-D-aspartate receptors (NMDARs). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/10719892; hal-00140070; https://hal.science/hal-00140070Test; https://hal.science/hal-00140070/documentTest; https://hal.science/hal-00140070/file/Roux_Supplisson_2000_Neuron_25_373-383.pdfTest; PUBMED: 10719892 |
| الإتاحة: | https://hal.science/hal-00140070Test https://hal.science/hal-00140070/documentTest https://hal.science/hal-00140070/file/Roux_Supplisson_2000_Neuron_25_373-383.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.88744C52 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |