Some Plant Defense Stimulators can induce IL-1β production in human immune cells in vitro
| العنوان: | Some Plant Defense Stimulators can induce IL-1β production in human immune cells in vitro |
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| المؤلفون: | Sébastien Aimé, Gabriele Sorci, Johanna Chluba, Jean-Louis Connat, David Wendehenne, Lény Teyssier, Olivier Lamotte |
| المساهمون: | Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Work supported by a FEDER grant Phytosafe, Présage 39703 from European Community, and by a grant from the Conseil Régional de Bourgogne (JCE funding)., Laffont, Rémi, Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement |
| المصدر: | Toxicology Reports Toxicology Reports, 2020, 7, pp.413-420. ⟨10.1016/j.toxrep.2020.02.013⟩ Toxicology Reports, Vol 7, Iss, Pp 413-420 (2020) Toxicology Reports, Elsevier, 2020, 7, pp.413-420. ⟨10.1016/j.toxrep.2020.02.013⟩ |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | [SDV.IMM] Life Sciences [q-bio]/Immunology, Health, Toxicology and Mutagenesis, medicine.medical_treatment, XTT, 010501 environmental sciences, A vision for the future of pesticide toxicology, Toxicology, 01 natural sciences, Peripheral blood mononuclear cell, Microbiology, 03 medical and health sciences, Laminarin, chemistry.chemical_compound, 0302 clinical medicine, Immune system, lcsh:RA1190-1270, medicine, Plant defense against herbivory, Pesticides, Cytotoxicity, lcsh:Toxicology. Poisons, 0105 earth and related environmental sciences, Inflammation, Cell metabolic activity, Innate immune system, Danio rerio, ASM, acyl-benzolar-S-methyl, Zebra fish, PBMC, Plant Defense Stimulator, In vitro, 3. Good health, PPP, Plant Protection product, Cytokine, chemistry, PDS, Plant Defense Stimulator, PBMC, peripheral blood mononuclear cells, IL-1β, [SDV.IMM]Life Sciences [q-bio]/Immunology, 030217 neurology & neurosurgery |
| الوصف: | 8 pages; International audience; Among Plant Protection Products (PPP), a new emerging category of pesticides act by stimulating plant defense in order to improve plant resistance against microbial pathogens. Given that these compounds, the so-called Plant Defense Stimulators (PDS) act on innate immunity, we tested, using an in vitro approach on human mononuclear leucocytes (PBMC), the potential toxicity (XTT assay) and inflammatory effects (production of IL-1β) of 4 PPP belonging to different chemical families. We found that two products (LBG-01F34® and Regalis®) did not induce any cytotoxicity or IL-1 β production. The product BION-50 WG®, that contains Acibenzolar-S-methyl (ASM) and silica particles did not present any cytotoxicity but induced a significant increase in the production of the inflammatory cytokine IL-1 β. Finally, Vacciplant® that contains laminarin, was highly cytotoxic and pro-inflammatory. It induced a strong production of IL-1 β when used at a concentration in the culture medium, as low as 0.02 mg/mL. We also tested the potential toxic effect of these 4 PPP on 4 days old zebra fish larvae. After 24 h of exposure, our results indicate that Vacciplant® induced zebra fish larvae mortality at concentration of 20 μg/mL. LBG did not induced significant mortality at concentrations up to 1 mg/mL whereas Regalis was lethal for 0,3 mg/mL concentrations and BION-50 WG began to induce mortality at 2,5 mg/mL. Our results indicate possible effects of PDS on IL-1β production in human cells and fish survival, calling for more studies on the potential noxious side effects of these compounds. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2214-7500 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b855f453faee38753756b2cca2227f9Test http://europepmc.org/articles/PMC7047147Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7b855f453faee38753756b2cca2227f9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22147500 |
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