المؤلفون: Dai Takeuchi, Takahiro Inaishi, Noriyuki Miyajima, Masahiro Shibata, Toyone Kikumori

المصدر: Surgery. 167(2):385-389

مصطلحات موضوعية: Adult, Male, Pathology, medicine.medical_specialty, Adolescent, medicine.medical_treatment, Adipose tissue, 030230 surgery, Parathyroid Glands, 03 medical and health sciences, chemistry.chemical_compound, Young Adult, 0302 clinical medicine, Lactate dehydrogenase, medicine, Humans, Aspartate Aminotransferases, Lymph node, Aged, Retrospective Studies, Aged, 80 and over, Parathyroidectomy, Frozen section procedure, L-Lactate Dehydrogenase, business.industry, Thyroid, Thyroidectomy, Middle Aged, Autotransplantation, medicine.anatomical_structure, chemistry, 030220 oncology & carcinogenesis, Surgery, Female, Lymph, business, hormones, hormone substitutes, and hormone antagonists

الوصف: Background: Identification of parathyroid tissue during surgery is necessary for its preservation in situ or for autotransplantation to avoid postoperative hypoparathyroidism. Frozen sections are the gold standard for distinguishing parathyroid tissue from other tissues during thyroidectomy. Although frozen sections are very accurate, they are costly and require pathologists and technical staff. Parathyroid tissue is rich in mitochondria, which harbor Krebs-cycle enzymes such as aspartate aminotransferase. In contrast, lactate dehydrogenase is expressed ubiquitously. These 2 enzymes are measured routinely as “leaked” enzymes. We hypothesized that the aspartate aminotransferase–to–lactate dehydrogenase ratio in suspended tissue could distinguish parathyroid tissue from other tissues. Methods: We analyzed 94 specimens (43 parathyroid, 19 thyroid cancers, 13 normal lymph nodes, 10 adipose, 6 thyroid, and 3 miscellaneous tissues) from 55 patients who underwent thyroid or parathyroid surgery between March 2018 and June 2019 in our institution. Trace amounts of remnant parathyroid tissue from autotransplantation specimens were suspended in 1 mL of normal saline and measured for aspartate aminotransferase and lactate dehydrogenase. Approximately 1 mm3 of apparently distinct tissue minced by scissors (eg, thyroid gland, metastatic lymph node, etc) or washouts of needles used for preoperative aspiration biopsy were also measured for comparison. Results: The aspartate aminotransferase–to–lactate dehydrogenase ratios in suspended parathyroid tissue specimens were consistently greater than those of other tissues (P < .001, Mann-Whitney test); 0.27 was the optimal cutoff value with 100% sensitivity and specificity. Conclusion: This method distinguished parathyroid tissue quickly and conveniently from other tissues intraoperatively with minimum cost and without dedicated pathologic staff. This methodology may serve useful in decreasing the incidence of postoperative hypoparathyroidism, especially in settings with limited access to pathologists.
ファイル公開：2021-02-01

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f43a7cd908ed564dffafe237659841f1Test
https://nagoya.repo.nii.ac.jp/records/30104Test

المؤلفون: Arturo Panduro, Jorge L Trujillo-Ochoa, Mauricio Realpe-Quintero, Nora A Fierro, Sonia Roman, Oliver Viera-Segura, Alexis Jose-Abrego, Karina Gonzalez-Aldaco

المصدر: Annals of Hepatology, Vol 18, Iss 4, Pp 571-577 (2019)

مصطلحات موضوعية: Liver Cirrhosis, Male, Cirrhosis, Transient elastography, viruses, Specialties of internal medicine, Disease, Chronic liver disease, medicine.disease_cause, Polymerase Chain Reaction, 0302 clinical medicine, Hepatitis E virus, Reverse Transcriptase Polymerase Chain Reaction, Liver Diseases, virus diseases, Alanine Transaminase, gamma-Glutamyltransferase, General Medicine, Middle Aged, Hepatitis E, RC581-951, 030220 oncology & carcinogenesis, Elasticity Imaging Techniques, RNA, Viral, Female, 030211 gastroenterology & hepatology, Adult, Genotype, Hepatitis C virus, Liver fibrosis, 03 medical and health sciences, medicine, Humans, Aspartate Aminotransferases, Mexico, Genotyping, HEV-infection, Retrospective Studies, Hepatitis B virus, L-Lactate Dehydrogenase, Hepatology, Platelet Count, business.industry, Bilirubin, medicine.disease, Virology, digestive system diseases, Chronic Disease, business

الوصف: Introduction and Objectives To characterize the virological features of hepatitis E virus (HEV) in serum from patients exhibiting chronic liver damage. Methods A data-base of 513 unrelated individuals from West-Mexico with liver-disease determined by clinical and biochemical tests and transient elastography between 2011 and 2016 were retrospectively analyzed. According to infectious etiologies, patients were classified as hepatitis B virus (HBV)-, hepatitis C virus (HCV)-infected patients, and patients exhibiting chronic liver damage with non-identified infectious etiological agent (NIIEA). Available serum samples from NIIEA-patients were tested by RT-nPCR for the presence of HEV-RNA and partially sequenced for genotyping. Results Out of the 513 cases, 5.85% were patients infected with HBV, 67.64% with HCV, and 26.51% were NIIEA-patients. Among 76 available samples from NIIEA-cases, 30.26% tested positive for HEV-RNA. Twelve (15.79%) partial HEV sequences allowed phylogenetic analysis, revealing the classification of HEV as HEV-Gt3. Advanced fibrosis (F3–F4 stage) was found in a 26.1% of patients with HEV-active infection. Conclusion Although HCV is the main infectious agent related to chronic liver disease in Mexico, liver damage without an infectious etiology is common. Our findings reveal that an elevated rate of chronic liver disease might be represented by autochthonous infection of HEV-Gt3, whose detection makes Mexico unique in Latin-America with the circulation of HEV strains belonging to three genotypes (Gt1, Gt2, and Gt3). Thus, HEV infection should be a matter of health concern, and mandates for HEV screening to properly handle this commonly undiagnosed disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1738d251386e127740585235dee04cc9Test
http://www.sciencedirect.com/science/article/pii/S1665268119300614Test

المؤلفون: Ozkan Isik, İlkay Doğan

المصدر: Biomedical Journal

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Statistical difference, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lower body, Internal medicine, Lactate dehydrogenase, One-repetition maximum, medicine, Humans, Skeletal muscle damage, Aspartate Aminotransferases, Creatine kinase, Muscle, Skeletal, Exercise, Creatine-Kinase, Adaptations, biology, L-Lactate Dehydrogenase, business.industry, Resistance training, Repeated measures design, Alanine Transaminase, Resistance Training, 030229 sport sciences, General Medicine, Unilateral, Bilateral, Resistance exercise, chemistry, Squat, biology.protein, Cardiology, Original Article, Strength, business, 030217 neurology & neurosurgery, Biomarkers

الوصف: Background: It is known that different intensity exercises create skeletal muscle damage at different levels. The purpose of the study was to compare effects of bilateral or unilateral lower body resistance exercise on markers of skeletal muscle damage. Methods: The Brzycki Formula was used to calculate participants' one repetition maximum strength for each movement and limb, separately. Blood samples were obtained before exercise, immediately after exercise, and 30 min after exercise for both types of exercise. Creatine Kinase (CK), Lactate Dehydrogenase (LDH), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT) levels were analyzed. Data was analyzed using two-way repeated measures ANOVA. Results: When CK, AST, and ALT levels were compared according to time points, a statistical difference was found (p < 0.05). Furthermore, it was revealed that LDH levels were statistically significant according to exercise types (p < 0.05). Conclusion: Unilateral lower body resistance exercise caused higher skeletal muscle damage than the bilateral lower body resistance exercise. This result suggests that unilateral lower body resistance exercise should be preferred for short preparation period as opposed to bilateral lower body resistance exercise. Afyon Kocatepe University Scientific Research Coordination OfficeAfyon Kocatepe University [17.KARIYER.106] This study was supported by the Afyon Kocatepe University Scientific Research Coordination Office with the number of 17.KARIYER.106.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92c8cb38d54525f7dd9446ea021e9739Test
https://hdl.handle.net/20.500.14002/113Test

المؤلفون: Adriana Ferlazzo, Katiuska Satué, Esterina Fazio, Pietro Medica

مصطلحات موضوعية: medicine.medical_specialty, 040301 veterinary sciences, Bilirubin, Sodium, chemistry.chemical_element, Calcium, digestive system, Biochemistry, 0403 veterinary science, chemistry.chemical_compound, Ovarian Follicle, Lactate dehydrogenase, Internal medicine, mare, medicine, Animals, Aspartate Aminotransferases, Horses, Blood urea nitrogen, Triglycerides, Equine, Chemistry, 0402 animal and dairy science, Albumin, 04 agricultural and veterinary sciences, Biochemistry, follicular fluid, mare, proteinogram, 040201 dairy & animal science, Follicular fluid, follicular fluid, proteinogram, Cholesterol, Endocrinology, Alkaline phosphatase, Female

الوصف: The aim of this study was to verify the existence of possible cross-talk between biochemical contents of follicular fluid (FF) and systemic concentrations according to the follicular development of the metabolites: glucose (GLU), lactate (LACT), cholesterol (CHOL), triglycerides (TG), total bilirubin (T BIL), blood urea nitrogen (BUN), and creatinine (CREAT); enzymatic activities: gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and aspartate aminotransferase (AST); electrolytes: calcium (Ca), phosphorus (P), sodium (Na), chloride (Cl), potassium (K), magnesium (Mg), and iron (Fe); total proteins (TP) and their fractions: albumin (ALB), α1-, α2-, β-, and γ-globulins (GLOB) of FF and blood strain and their correlations with follicular size in cycling mares. Systemic concentrations of GLU, T BIL, BUN, Fe, TP, ALB, α-1, and α-2 and δ-GLOB and of ALP, GGT, and AST activities were higher than in the FF (P.05); LACT, CHOL, and TG were higher in FF than systemic ones (P.05). Glucose, CHOL, TG, LACT, and T BIL were higher in large follicles than in medium and small follicles (P.05); however, BUN, Fe, ALP, and AST were lower in large follicles than in medium or small follicles (P.05). Alkaline phosphatase, GGT, and AST activities decreased in medium and large follicles compared with small follicles (P.05). These results suggest that the metabolic, enzymatic, electrolytic, and protein composition of FF of growing follicles could occur according to the bloodstream changes; hence, it is possible to presume that the nutritional environment of oocyte and follicular cells could improve the clinical diagnoses of infertility in the mare.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3034ccc41a07511e38d0f6097c7e9dbTest
http://hdl.handle.net/11570/3146260Test

المؤلفون: Francisco Javier López-Román, Domingo J. Ramos-Campo, Fulgencio Soto-Méndez, Jacobo Á. Rubio-Arias, Pedro E. Alcaraz, Vicente Ávila-Gandía

المصدر: RIUCAM. Repositorio Institucional de la Universidad Católica San Antonio de Murcia
instname

مصطلحات موضوعية: Adult, Male, Time Factors, Adolescent, LDH, Physiology, Experimental and Cognitive Psychology, Inflammation, Muscle damage, Hematocrit, Running, C-reactive protein, 03 medical and health sciences, Behavioral Neuroscience, Young Adult, 0302 clinical medicine, Medicine, Humans, 0501 psychology and cognitive sciences, 050102 behavioral science & comparative psychology, Aspartate Aminotransferases, Hematocrit and hemoglobin, Creatine Kinase, Ultra endurance, Transaminases, Inflammation biomarkers, biology, medicine.diagnostic_test, L-Lactate Dehydrogenase, business.industry, Athletes, Myoglobin, 05 social sciences, Troponin I, Alanine Transaminase, biology.organism_classification, Blood Cell Count, Uric Acid, Cross-Sectional Studies, CK, Creatinine, biology.protein, Physical Endurance, Hemoglobin, medicine.symptom, business, 030217 neurology & neurosurgery, Biomarkers

الوصف: The aims of this study were 1) to describe the effects of a 54 km and 111 km ultra-endurance mountain race on the biomarkers of muscle damage and inflammation, 2) to compare the effects between the two races regarding the biomarkers of muscle damage and inflammation. Sixteen ultra-endurance amateur runners volunteered to participate in this study. Ten runners completed a 54 km race (Group 1; age: 27.0 ± 5.7; height: 179.5 ± 5.8 cm; and body mass: 77.3 ± 10.7 kg) and six completed a 111 km race (Group 2; age: 30.5 ± 8.0; height: 179.4 ± 5.5 cm; and body mass: 76.2 ± 9.4 kg). Blood samples were taken at five different points during the investigation, 24 h before the race, immediately post-race, and again at 24, 48, and 72 h after the race. There were increases in leukocyte (Group 1: p ≤ .001, ES = 2.8; Group 2: p = .001, ES = 3.5) and platelet concentrations (Group 1: p ≤ .001, ES = 2.3; Group 2: p = .02, ES = 1.7) post-races. Significant inter-race differences were also observed in leukocyte at 72 h (Group 1: 5.5 ± 0.9, Group 2: 4.2 ± 0.9, p = .012, ES = 1.5). Erythrocytes, hematocrit and hemoglobin concentration decreased after 54 km and 111 km races at 24, 48 and 72 h (p ≤.001, ES = 2.0–3.18). Serum uric acid concentration increased after the 54 km race (pre = 4.9 ± 1.2 – post = 7.3 ± 1.0 8 mg/dl; p ≤ .001, ES = 2.4), and also the 111 km race (pre = 5.3 ± 0.9 – post = 6.7 ± 0.8 mg/dl; p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36e1c032a1573e5c5b08acb38019ac24Test
http://hdl.handle.net/10952/3664Test

المؤلفون: R. Vivo, A. Molina, S. Agüera, P. Tovar, Estrella I. Agüera, Begoña M. Escribano, R. Santisteban, Mercedes Valera, M.D. Rubio

المصدر: Animal, Vol 7, Iss 9, Pp 1414-1422 (2013)

مصطلحات موضوعية: Male, Veterinary medicine, Aspartate transaminase, Physiology, heritability, Models, Biological, SF1-1100, chemistry.chemical_compound, Reference Values, Lactate dehydrogenase, Physical Conditioning, Animal, Animals, Aspartate Aminotransferases, Horses, Lactic Acid, Treadmill, Creatinine, Likelihood Functions, Hematologic Tests, biochemical reference limits, Andalusian horse, biology, L-Lactate Dehydrogenase, Horse, Blood Proteins, Heritability, Blood Cell Count, Uric Acid, Animal culture, chemistry, Hematocrit, treadmill, biology.protein, Exercise Test, Uric acid, Animal Science and Zoology, haematological reference limits, Purebred

الوصف: The novel aim of this study was to describe the reference values of different haematological and biochemical parameters in the Spanish purebred horse (Andalusian, SPB) in each of the stages of a programmed exercise on a treadmill system, and to establish heritability and genetic correlations for these haematological and biochemical parameters. For this, 94 young SPB male horses (4.22 ± 2.27 years old) were used. An increasing intensity exercise test at 4, 5, 6 and 7 m/s was carried out on a treadmill (6% inclination). Total red blood cells, total white blood cells, neutrophils and lymphocytes counts; haematocrit, haemoglobin, lactate, uric acid, creatinine and total plasma proteins concentrations and aspartate transaminase, lactate dehydrogenase, creatine-quinase activities were determined. To conclude: (i) the reference values for each parameter were determined for each of the exercise test stages (ii) all the parameters analysed manifested a medium-high heritability and a high repeatability. These results will, in the near future, determine the measuring guidelines for improving the SPB horse's athletic ability on an objective treadmill system and for selecting these animals in response to those parameters.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64501e462988adbd1f7412017cb1c527Test
http://www.sciencedirect.com/science/article/pii/S1751731113000955Test

المؤلفون: Masahiro Yamasaki, Madoka Yonemura, Hiromitsu Orima, Osamu Yamato, Tomoya Asano, Daisuke Hasegawa, Hiroyuki Satoh, Toshiro Arai, Toyofumi Yamauchi, Yoshimitsu Maede

المصدر: Brain Research. 1133(1):200-208

مصطلحات موضوعية: Pathology, medicine.medical_specialty, GM1 gangliosidosis, Prednisolone, Enolase, Glucocorticoid therapy, Anti-Inflammatory Agents, Aspartate transaminase, G(M1) Ganglioside, Aspartate aminotransferase, chemistry.chemical_compound, Cerebrospinal fluid, Dogs, Predictive Value of Tests, Lactate dehydrogenase, Lysosomal storage disease, medicine, Animals, Aspartate Aminotransferases, Molecular Biology, Gangliosidosis, GM1, biology, L-Lactate Dehydrogenase, General Neuroscience, Cerebrospinal fluid biomarker, Brain, Cerebrospinal Fluid Proteins, Myelin Basic Protein, medicine.disease, Myelin basic protein, Up-Regulation, Disease Models, Animal, Treatment Outcome, chemistry, Phosphopyruvate Hydratase, Immunology, Nerve Degeneration, biology.protein, lipids (amino acids, peptides, and proteins), Neurology (clinical), Canine model, Glucocorticoid, Biomarkers, Developmental Biology, medicine.drug

الوصف: The present study investigated cerebrospinal fluid (CSF) biomarkers for estimating degeneration of the central nervous system (CNS) in experimental dogs with GM1 gangliosidosis and preliminarily evaluated the efficacy of long-term glucocorticoid therapy for GM1 gangliosidosis using the biomarkers identified here. GM1 gangliosidosis, a lysosomal storage disease that affects the brain and multiple systemic organs, is due to an autosomal recessively inherited deficiency of acid beta-galactosidase activity. Pathogenesis of GM1 gangliosidosis may include neuronal apoptosis and abnormal axoplasmic transport and inflammatory response, which are perhaps consequent to massive neuronal storage of GM1 ganglioside. In the present study, we assessed some possible CSF biomarkers, such as GM1 ganglioside, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), neuron-specific enolase (NSE) and myelin basic protein (MBP). Periodic studies demonstrated that GM1 ganglioside concentration, activities of AST and LDH, and concentrations of NSE and MBP in CSF were significantly higher in dogs with GM1 gangliosidosis than those in control dogs, and their changes were well related with the months of age and clinical course. In conclusion, GM1 ganglioside, AST, LDH, NSE and MBP could be utilized as CSF biomarkers showing CNS degeneration in dogs with GM1 gangliosidosis to evaluate the efficacy of novel therapies proposed for this disease. In addition, we preliminarily treated an affected dog with long-term oral administration of prednisolone and evaluated the efficacy of this therapeutic trial using CSF biomarkers determined in the present study. However, this treatment did not change either the clinical course or the CSF biomarkers of the affected dog, suggesting that glucocorticoid therapy would not be effective for treating GM1 gangliosidosis.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fb47b7fc42b35ee27edc49117f2e8d9Test
http://hdl.handle.net/2115/20150Test

المؤلفون: Belda Erkmen, Dürdane Kolankaya, Abbas Güngördü

المساهمون: Sabire Yazıcı Fen Edebiyat Fakültesi, [Gungordu, Abbas] Inonu Univ, Sci & Arts Fac, Dept Biol, Lab Environm Toxicol, TR-44280 Malatya, Turkey -- [Erkmen, Belda] Aksaray Univ, Sci & Arts Fac, Dept Biol, TR-68100 Aksaray, Turkey -- [Kolankaya, Durdane] Hacettepe Univ, Dept Biol, Fac Sci, TR-06532 Ankara, Turkey, Gungordu, Abbas -- 0000-0001-7770-0660

مصطلحات موضوعية: Delta, Carps, Meric Delta, Turkey, Health, Toxicology and Mutagenesis, Acid Phosphatase, Zoology, Biology, Toxicology, Cyprinus Carpio, Cyprinus, Carboxylesterase, Enzyme Activity, Common carp, chemistry.chemical_compound, Rivers, Lactate dehydrogenase, Metals, Heavy, Biomonitoring, Animals, Aspartate Aminotransferases, Carp, Glutathione Transferase, Pharmacology, L-Lactate Dehydrogenase, General Medicine, Biomarker, biology.organism_classification, Meriç Delta, chemistry, Liver, Xenobiotic, Biomarkers, Water Pollutants, Chemical, Environmental Monitoring

الوصف: WOS: 000302894400008
PubMed: 22387355
The aim of this study is to examine the activity of several biomarkers in carp, Cyprinus carpi L., to determine their response to xenobiotics, such as organochlorine pesticides (OCPs), in the Meric Delta. Fish were collected from contaminated sites and from areas regarded as relatively less contaminated in four sampling periods. Hepatic glutathione S-transferase (GST), carboxylesterase (CaE), lactate dehydrogenase, aspartate aminotransferase, and acid phosphatase activities were measured as biomarkers in the fish. For all fish, the condition factor and hepatosomatic index, were calculated to determine the condition of the fish. The results of this study indicated that the mean GST activity showed an increase in fish from the Meric-Ergene junction site and a decrease in Enez site with respect to fish from Meric site. Furthermore, the study shows that spatial and temporal changes of biomarkers such as GST and CaE might be useful for the assessment of environmental contamination in the Meric Delta. (C) 2012 Elsevier B.V. All rights reserved.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::940dcec7281bef347d930b9b0b3274e4Test
https://hdl.handle.net/20.500.12451/2100Test

المؤلفون: G. Lippi, F. Schena, M. Montagnana, G. L. Salvagno, G. C. Guidi, G. Banfi

المساهمون: G. Lippi, F. Schena, M. Montagnana, G.L. Salvagno, G. Banfi, G.C. Guidi

مصطلحات موضوعية: Liver Diseases, Running, Humans, Prognosis, Myoglobin, Aspartate Aminotransferases, Liver Function Tests, Physical Endurance, L-Lactate Dehydrogenase, Prospective Studies, Creatine Kinase, Adult, MB Form, Bilirubin, Follow-Up Studies, Middle Aged, Biological Markers, Male, Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica

الوصف: While the promotion of health-related fitness is thereby widespread, less focus is currently being given on the biological influence that physical activity might exert on results of laboratory testing. As such, this study was undertaken to assess the kinetics of liver injury markers following physical exercise.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/21925040; info:eu-repo/semantics/altIdentifier/wos/WOS:000295455000002; volume:22; issue:5; firstpage:e36; lastpage:e38; journal:EUROPEAN JOURNAL OF INTERNAL MEDICINE; http://hdl.handle.net/2434/179325Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-80052967100

الإتاحة: https://doi.org/10.1016/j.ejim.2011.02.007Test
http://hdl.handle.net/2434/179325Test

المؤلفون: Miguel A. Miranda, M. J. Gomez-L., Isabel M. Morera, J.V. Castell, V. Mirabet

المصدر: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Scopus-Elsevier

مصطلحات موضوعية: Male, Magnetic Resonance Spectroscopy, Chemical Phenomena, Light, Cell Survival, Pharmaceutical Science, Benorilate, chemistry.chemical_compound, Benzophenones, Transacylation, QUIMICA ORGANICA, Lactate dehydrogenase, Albumins, medicine, Cell Adhesion, Animals, Aspartate Aminotransferases, Cells, Cultured, Photolysis, Glycogen, L-Lactate Dehydrogenase, Spectrum Analysis, Albumin, Gluconeogenesis, Fructose, Rats, Inbred Strains, Salicylates, Rats, Chemistry, chemistry, Biochemistry, Liver, Cell culture, Toxicity, medicine.drug

الوصف: [EN] The photodegradation of benorylate [4¿-(acetamido)phenyl-2-acetoxybenzoate], a drug frequently used in rheumatoid arthritis therapy, has been examined under different sets of experimental conditions. Several photoproducts have been isolated and identified on the basis of their IR, NMR, and MS spectra. The most significant photochemical process is the photo-Fries rearrangement of benorylate, leading to 5-acetamido-2¿-acetoxy-2-hydroxybenzophenone (1). This compound undergoes a rapid transacylation to the isomeric 5¿-acetamido-2¿-acetoxy-2-hydroxybenzophenone (2). A primary culture of rat hepatocytes has been used to evaluate the possible toxicity of these two benzophenones, keeping in mind the following criteria: leakage of cytosolic enzymes, attachment index to culture plates, gluconeogenesis from lactate and fructose, glycogen balance, and albumin synthesis. At the concentrations assayed, neither of the two major photoproducts of benorylate (benzophenones 1 and 2) had significant toxic effects on liver cells in culture.
Financial support Comisión Asesora de Investigación Científica y Técnica (grant no.1325) is gratefully acknowledged.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04eabe0be09689a4b774ea215d1424e4Test