دورية أكاديمية
The carboxyl-terminal sequence of bim enables bax activation and killing of unprimed cells
| العنوان: | The carboxyl-terminal sequence of bim enables bax activation and killing of unprimed cells |
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| المؤلفون: | Xiaoke Chi, Dang Nguyen, James M Pemberton, Elizabeth J Osterlund, Qian Liu, Hetal Brahmbhatt, Zhi Zhang, Jialing Lin, Brian Leber, David W Andrews |
| المصدر: | eLife, Vol 9 (2020) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | primary murine neurons, MEF cells, BMK cells, apoptosis, Bcl-2 family proteins, mitochondrial outer membrane permeabilization, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The Bcl-2 family BH3 protein Bim promotes apoptosis at mitochondria by activating the pore-forming proteins Bax and Bak and by inhibiting the anti-apoptotic proteins Bcl-XL, Bcl-2 and Mcl-1. Bim binds to these proteins via its BH3 domain and to the mitochondrial membrane by a carboxyl-terminal sequence (CTS). In cells killed by Bim, the expression of a Bim mutant in which the CTS was deleted (BimL-dCTS) triggered apoptosis that correlated with inhibition of anti-apoptotic proteins being sufficient to permeabilize mitochondria isolated from the same cells. Detailed analysis of the molecular mechanism demonstrated that BimL-dCTS inhibited Bcl-XL but did not activate Bax. Examination of additional point mutants unexpectedly revealed that the CTS of Bim directly interacts with Bax, is required for physiological concentrations of Bim to activate Bax and that different residues in the CTS enable Bax activation and binding to membranes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/44525Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.44525 |
| الوصول الحر: | https://doaj.org/article/3930186c3fd04ad4a33907088e2464c2Test |
| رقم الانضمام: | edsdoj.3930186c3fd04ad4a33907088e2464c2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.44525 |