دورية أكاديمية
Functional heterogeneity of lymphocytic patterns in primary melanoma dissected through single-cell multiplexing
| العنوان: | Functional heterogeneity of lymphocytic patterns in primary melanoma dissected through single-cell multiplexing |
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| المؤلفون: | Francesca Maria Bosisio, Asier Antoranz, Yannick van Herck, Maddalena Maria Bolognesi, Lukas Marcelis, Clizia Chinello, Jasper Wouters, Fulvio Magni, Leonidas Alexopoulos, Marguerite Stas, Veerle Boecxstaens, Oliver Bechter, Giorgio Cattoretti, Joost van den Oord |
| المصدر: | eLife, Vol 9 (2020) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | melanoma, multiplex, tumor-infiltrating lymphocytes, TILs, microenvironment, brisk, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | In melanoma, the lymphocytic infiltrate is a prognostic parameter classified morphologically into ‘brisk’, ‘non-brisk’ and ‘absent’ entailing a functional association that has never been proved. Recently, it has been shown that lymphocytic populations can be very heterogeneous, and that anti-PD-1 immunotherapy supports activated T cells. Here, we characterize the immune landscape in primary melanoma by high-dimensional single-cell multiplex analysis in tissue sections (MILAN technique) followed by image analysis, RT-PCR and shotgun proteomics. We observed that the brisk and non-brisk patterns are heterogeneous functional categories that can be further sub-classified into active, transitional or exhausted. The classification of primary melanomas based on the functional paradigm also shows correlation with spontaneous regression, and an improved prognostic value when compared to that of the brisk classification. Finally, the main inflammatory cell subpopulations that are present in the microenvironment associated with activation and exhaustion and their spatial relationships are described using neighbourhood analysis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/53008Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.53008 |
| الوصول الحر: | https://doaj.org/article/5f94f70b8b1b46cf85d03da3a1097f62Test |
| رقم الانضمام: | edsdoj.5f94f70b8b1b46cf85d03da3a1097f62 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.53008 |