دورية أكاديمية
Increasing evidence of mechanical force as a functional regulator in smooth muscle myosin light chain kinase
| العنوان: | Increasing evidence of mechanical force as a functional regulator in smooth muscle myosin light chain kinase |
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| المؤلفون: | Fabian Baumann, Magnus Sebastian Bauer, Martin Rees, Alexander Alexandrovich, Mathias Gautel, Diana Angela Pippig, Hermann Eduard Gaub |
| المصدر: | eLife, Vol 6 (2017) |
| بيانات النشر: | eLife Sciences Publications Ltd |
| سنة النشر: | 2017 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | smooth muscle myosin light chain kinase, force-activation, cytoskeleton, auto-inhibition, single-molecule force spectroscopy, atomic force microscopy, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Mechanosensitive proteins are key players in cytoskeletal remodeling, muscle contraction, cell migration and differentiation processes. Smooth muscle myosin light chain kinase (smMLCK) is a member of a diverse group of serine/threonine kinases that feature cytoskeletal association. Its catalytic activity is triggered by a conformational change upon Ca2+/calmodulin (Ca2+/CaM) binding. Due to its significant homology with the force-activated titin kinase, smMLCK is suspected to be also regulatable by mechanical stress. In this study, a CaM-independent activation mechanism for smMLCK by mechanical release of the inhibitory elements is investigated via high throughput AFM single-molecule force spectroscopy. The characteristic pattern of transitions between different smMLCK states and their variations in the presence of different substrates and ligands are presented. Interaction between kinase domain and regulatory light chain (RLC) substrate is identified in the absence of CaM, indicating restored substrate-binding capability due to mechanically induced removal of the auto-inhibitory regulatory region. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/26473Test; https://doaj.org/toc/2050-084XTest; e26473; https://doaj.org/article/0909c6d931614472b45523df1eba52f6Test |
| DOI: | 10.7554/eLife.26473 |
| الإتاحة: | https://doi.org/10.7554/eLife.26473Test https://doaj.org/article/0909c6d931614472b45523df1eba52f6Test |
| رقم الانضمام: | edsbas.7B15C2EF |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.26473 |