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Induction of homologous recombination between sequence repeats by the activation induced cytidine deaminase (AID) protein

التفاصيل البيبلوغرافية
العنوان: Induction of homologous recombination between sequence repeats by the activation induced cytidine deaminase (AID) protein
المؤلفون: Jean-Marie Buerstedde, Noel Lowndes, David G Schatz
المصدر: eLife, Vol 3 (2014)
بيانات النشر: eLife Sciences Publications Ltd, 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: AID, homologous recombination, DNA repair, Medicine, Science, Biology (General), QH301-705.5
الوصف: The activation induced cytidine deaminase (AID) protein is known to initiate somatic hypermutation, gene conversion or switch recombination by cytidine deamination within the immunoglobulin loci. Using chromosomally integrated fluorescence reporter transgenes, we demonstrate a new recombinogenic activity of AID leading to intra- and intergenic deletions via homologous recombination of sequence repeats. Repeat recombination occurs at high frequencies even when the homologous sequences are hundreds of bases away from the positions of AID-mediated cytidine deamination, suggesting DNA end resection before strand invasion. Analysis of recombinants between homeologous repeats yielded evidence for heteroduplex formation and preferential migration of the Holliday junctions to the boundaries of sequence homology. These findings broaden the target and off-target mutagenic potential of AID and establish a novel system to study induced homologous recombination in vertebrate cells.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
العلاقة: https://elifesciences.org/articles/03110Test; https://doaj.org/toc/2050-084XTest
DOI: 10.7554/eLife.03110
الوصول الحر: https://doaj.org/article/b98dd0701533462b8301536e36ba1e92Test
رقم الانضمام: edsdoj.b98dd0701533462b8301536e36ba1e92
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.03110