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Telomere length regulation by Rif1 protein from Hansenula polymorpha

التفاصيل البيبلوغرافية
العنوان: Telomere length regulation by Rif1 protein from Hansenula polymorpha
المؤلفون: Alexander N Malyavko, Olga A Petrova, Maria I Zvereva, Vladimir I Polshakov, Olga A Dontsova
المصدر: eLife, Vol 11 (2022)
بيانات النشر: eLife Sciences Publications Ltd, 2022.
سنة النشر: 2022
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: telomere, telomerase, Hansenula polymorpha, genome stability, DNA replication, Rif1, Medicine, Science, Biology (General), QH301-705.5
الوصف: Rif1 is a large multifaceted protein involved in various processes of DNA metabolism – from telomere length regulation and replication to double-strand break repair. The mechanistic details of its action, however, are often poorly understood. Here, we report functional characterization of the Rif1 homologue from methylotrophic thermotolerant budding yeast Hansenula polymorpha DL-1. We show that, similar to other yeast species, H. polymorpha Rif1 suppresses telomerase-dependent telomere elongation. We uncover two novel modes of Rif1 recruitment at H. polymorpha telomeres: via direct DNA binding and through the association with the Ku heterodimer. Both of these modes (at least partially) require the intrinsically disordered N-terminal extension – a region of the protein present exclusively in yeast species. We also demonstrate that Rif1 binds Stn1 and promotes its accumulation at telomeres in H. polymorpha.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
العلاقة: https://elifesciences.org/articles/75010Test; https://doaj.org/toc/2050-084XTest
DOI: 10.7554/eLife.75010
الوصول الحر: https://doaj.org/article/3450b54fe3ec4ecab4f33ed5f3e5ee04Test
رقم الانضمام: edsdoj.3450b54fe3ec4ecab4f33ed5f3e5ee04
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.75010