دورية أكاديمية
Mycobacterium tuberculosis induces decelerated bioenergetic metabolism in human macrophages
| العنوان: | Mycobacterium tuberculosis induces decelerated bioenergetic metabolism in human macrophages |
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| المؤلفون: | Bridgette M Cumming, Kelvin W Addicott, John H Adamson, Adrie JC Steyn |
| المصدر: | eLife, Vol 7 (2018) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | Mycobacterium tuberculosis, macrophages, bioenergetic metabolism, extracellular flux analysis, Mycobacterium bovis BCG, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | How Mycobacterium tuberculosis (Mtb) rewires macrophage energy metabolism to facilitate survival is poorly characterized. Here, we used extracellular flux analysis to simultaneously measure the rates of glycolysis and respiration in real time. Mtb infection induced a quiescent energy phenotype in human monocyte-derived macrophages and decelerated flux through glycolysis and the TCA cycle. In contrast, infection with the vaccine strain, M. bovis BCG, or dead Mtb induced glycolytic phenotypes with greater flux. Furthermore, Mtb reduced the mitochondrial dependency on glucose and increased the mitochondrial dependency on fatty acids, shifting this dependency from endogenous fatty acids in uninfected cells to exogenous fatty acids in infected macrophages. We demonstrate how quantifiable bioenergetic parameters of the host can be used to accurately measure and track disease, which will enable rapid quantifiable assessment of drug and vaccine efficacy. Our findings uncover new paradigms for understanding the bioenergetic basis of host metabolic reprogramming by Mtb. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/39169Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.39169 |
| الوصول الحر: | https://doaj.org/article/f415673efe5547e9af0ff253c87da097Test |
| رقم الانضمام: | edsdoj.f415673efe5547e9af0ff253c87da097 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.39169 |