مورد إلكتروني
Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate
| العنوان: | Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate |
|---|---|
| بيانات النشر: | eLife Sciences 2017-03-02 |
| تفاصيل مُضافة: | Counihan, Natalie A. Chisholm, Scott A. Bullen, Hayley E. Srivastava, Anubhav Sanders, Paul R. Jonsdottir, Thorey K. Weiss, Greta E. Ghosh, Sreejoyee Crabb, Brendan S. Creek, Darren J. Gilson, Paul R. de Koning-Ward, Tania F. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Plasmodium falciparum parasites, the causative agents of malaria, modify their host erythrocyte to render them permeable to supplementary nutrient uptake from the plasma and for removal of toxic waste. Here we investigate the contribution of the rhoptry protein RhopH2, in the formation of new permeability pathways (NPPs) in Plasmodium-infected erythrocytes. We show RhopH2 interacts with RhopH1, RhopH3, the erythrocyte cytoskeleton and exported proteins involved in host cell remodeling. Knockdown of RhopH2 expression in cycle one leads to a depletion of essential vitamins and cofactors and decreased de novo synthesis of pyrimidines in cycle two. There is also a significant impact on parasite growth, replication and transition into cycle three. The uptake of solutes that use NPPs to enter erythrocytes is also reduced upon RhopH2 knockdown. These findings provide direct genetic support for the contribution of the RhopH complex in NPP activity and highlight the importance of NPPs to parasite survival. |
| مصطلحات الفهرس: | inducible expression, P. berghei, cell biology, host cell remodeling, infectious disease, microbiology, rhoptry, Science & Technology, Life Sciences & Biomedicine, Biology, Life Sciences & Biomedicine - Other Topics, RED-BLOOD-CELLS, HUMAN MALARIA PARASITE, CLAG MULTIGENE FAMILY, RHOPTRY PROTEIN, PTEX COMPONENT, INFECTED ERYTHROCYTES, FUNCTIONAL-ANALYSIS, MEROZOITE INVASION, MAURERS CLEFTS, MEMBRANE, Journal Article |
| URL: | issn: 2050-084X eLife |
| الإتاحة: | Open access content. Open access content 2017, The Authors |
| ملاحظة: | 31 p. English |
| أرقام أخرى: | LD0 oai:dro.deakin.edu.au:DU:30092958 1002063393 |
| المصدر المساهم: | DEAKIN UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1002063393 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |