M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit
| العنوان: | M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit |
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| المؤلفون: | Jeremy Mortier, Stéphane Bertagnoli, Christelle Camus-Bouclainville, Sophie Blanié, Maxence Delverdier |
| المساهمون: | Institut National de la Recherche Agronomique - INRA (FRANCE), Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE) |
| المصدر: | Veterinary Research Veterinary Research, BioMed Central, 2009, 40 (1), pp.Inconnu. ⟨10.1051/vetres:2008049⟩ Veterinary Research 1 (40), Inconnu. (2009) |
| بيانات النشر: | EDP Sciences, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Rabbit, Virus Replication, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], 0303 health sciences, Myxomatosis, biology, Virulence, [SDV.BA]Life Sciences [q-bio]/Animal biology, 030302 biochemistry & molecular biology, Ankyrin repeat, Viral Load, Ankyrin Repeat, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, poxvirus, Poxvirus, [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Original Article, Rabbits, European rabbit, Gene Expression Regulation, Viral, Virulence Factors, rabbit, Context (language use), Myxoma virus, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Cell Line, Viral Proteins, 03 medical and health sciences, Myxomatosis, Infectious, biology.domesticated_animal, medicine, Animals, Poxviridae, Tropism, 030304 developmental biology, General Veterinary, F-Box Proteins, Wild type, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, biology.organism_classification, medicine.disease, Virology, virulence, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, Médecine vétérinaire et santé animal, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
| الوصف: | International audience; Myxoma virus (MYXV), a member of the Poxviridae family, is the agent responsible for myxomatosis, a fatal disease in the European rabbit (Oryctolagus cuniculus). MYXV has a linear double-stranded DNA genome that encodes several factors important for evasion from the host immune system. Among them, four ankyrin (ANK) repeat proteins were identified: M148R, M149R, M150R and M-T5. To date, only M150R and M-T5 were studied and characterized as critical virulence factors. This article presents the first characterization of M148R and M149R. Green Fluorescent Protein (GFP) fusions allowed us to localize them in a viral context. Whereas M149R is only cytoplasmic, interestingly, M148R is in part located in the nucleolus, a unique feature for an ANK repeat poxviral protein. In order to evaluate their implication in viral pathogenicity, targeted M148R, M149R, or both deletions were constructed in the wild type T1 strain of myxoma virus. In vitro infection of rabbit and primate cultured cells as well as primary rabbit cells allowed us to conclude that M148R and M149R are not likely to be implicated in cell tropism or host range functions. However, in vivo experiments revealed that they are virulence factors since after infection of European rabbits with mutant viruses, a delay in the onset of clinical signs, an increase of survival time and a dramatic decrease in mortality rate were observed. Moreover, histological analysis suggests that M148R plays a role in the subversion of host inflammatory response by MYXV. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0928-4249 1297-9716 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0957ca5e7db8a3adb779a6a1bc35e054Test https://oatao.univ-toulouse.fr/4016Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0957ca5e7db8a3adb779a6a1bc35e054 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09284249 12979716 |
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