دورية أكاديمية
Oxycodone ameliorates the inflammatory response induced by lipopolysaccharide in primary microglia
| العنوان: | Oxycodone ameliorates the inflammatory response induced by lipopolysaccharide in primary microglia |
|---|---|
| المؤلفون: | Jishi,Ye, Hong,Yan, Zhongyuan,Xia |
| بيانات النشر: | Dove Press |
| سنة النشر: | 2018 |
| المجموعة: | Dove Medical Press |
| مصطلحات موضوعية: | Journal of Pain Research |
| الوصف: | Jishi Ye,1 Hong Yan,2 Zhongyuan Xia1 1Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People’s Republic of China; 2Department of Anesthesiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, People’s Republic of China Background: Activation of microglia participates in a wide range of pathophysiological processes in the central nervous system. Some studies reported that oxycodone (6-deoxy-7,8-dehydro-14-hydroxy-3-O-methyl-6oxomorphine) could inhibit the overactivation of glial cells in rats’ spinal cords. In the present study, we observed the effect of oxycodone on inflammatory molecules and pathway in lipopolysaccharide (LPS)-stimulated primary microglia in rats. Materials and methods: Neonatal rats’ primary microglia were exposed to various concentrations (25, 50, 100 ng/mL) of oxycodone for 1 h after LPS stimulation for 24 h. The levels of pro-inflammatory mediators, IL-1β, TNF-α, and TGF-β1/smad2/3 signaling pathway were measured. The activation situation of microglia and the expression of TβR1 were observed by immunofluorescence. Results: Oxycodone at 25 ng/mL did not change the levels of proinflammatory molecules and TGF-β1/smad2/3 signaling pathway in primary microglia, which was increased by LPS. Oxycodone at 50 and 100 ng/mL could significantly suppress LPS-induced production of TNF-α and IL-1β and the expression of TNF-αmRNA, IL-1βmRNA, and TGF-β1/smad2/3 signaling pathway. Conclusion: These findings indicate that oxycodone, at relatively high clinically relevant concentration, can inhibit inflammatory response in LPS-induced primary microglia. The detailed mechanism needs to be investigated in future. Keywords: oxycodone, inflammatory, microglia |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | https://www.dovepress.com/oxycodone-ameliorates-the-inflammatory-response-induced-by-lipopolysac-peer-reviewed-fulltext-article-JPRTest |
| الإتاحة: | https://doi.org/10.2147/JPR.S160659Test https://www.dovepress.com/oxycodone-ameliorates-the-inflammatory-response-induced-by-lipopolysac-peer-reviewed-fulltext-article-JPRTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BB1D9991 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |